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Pregled bibliografske jedinice broj: 483541

Determination of mitotane and its metabolites in biological fluids by SPE-HPLC method

Mornar, Ana; Sertić, Miranda; Turk, Nikša; Koršić, Mirko; Nigović, Biljana
Determination of mitotane and its metabolites in biological fluids by SPE-HPLC method // Internation Scientific and Professional Conference 13th Ružička Days "Today Science - Tomorrow Industry" / Šubarić, Drago (ur.).
Osijek: Prehrambeno tehnološki fakultet Sveučilišta Josipa Jurja Strossmayera u Osijeku, 2010. (poster, međunarodna recenzija, sažetak, znanstveni)

CROSBI ID: 483541 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Determination of mitotane and its metabolites in biological fluids by SPE-HPLC method

Autori
Mornar, Ana ; Sertić, Miranda ; Turk, Nikša ; Koršić, Mirko ; Nigović, Biljana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Internation Scientific and Professional Conference 13th Ružička Days "Today Science - Tomorrow Industry" / Šubarić, Drago - Osijek : Prehrambeno tehnološki fakultet Sveučilišta Josipa Jurja Strossmayera u Osijeku, 2010

ISBN
978-953-7005-22-1

Skup
Internation Scientific and Professional Conference 13th Ružička Days "Today Science - Tomorrow Industry"

Mjesto i datum
Vukovar, Hrvatska, 16.09.2010. - 17.09.2010

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
mitotane; metabolites; Solid phase extraction; HPLC; biological fluids

Sažetak
Mitotane is the drug of choice for the treatment of adrenal cortical carcinoma and is used as a chemotherapeutic agent after resection. Its mechanism of action and pharmacokinetics are not fully known. To achieve optimal therapeutic concentration and to prevent dangerous side effects, it is very important to determinate the concentrations levels of mitotane and its metabolites in the body after administration. The aim of this work was to develop and validate a SPE-HPLC method for determination of mitotane and its metabolites in plasma, erythrocytes and urine of a patient with adrenal cortical carcinoma. Blood and urine samples were collected during 8 hours after administration of Lysodrena® (500 mg) pill. SPE conditions: Discovery DSC column, 500 mg, 3 mL (Supelco), preconditioned with 1 mL of methanol and 2 mL of acetonitrile. HPLC conditions: Symmetry C18 column (Waters), 150 × 4.6 mm ; 3.5 μm. Mobile phase: acetonitrile : MiliQ water : formic acid = 90 : 10 : 0.1 ; flow rate 1 mL/min ; DAD detector λmax 230 nm. Method was validated according to ICH guidelines and following parameters were examined: selectivity, linearity, limit of detection and determination, accuracy, precision and robustness. The proposed method was successfully applied for determination of mitotane and its metabolites in plasma, erythrocytes and urine.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti, Farmacija



POVEZANOST RADA

Projekti:
006-0061117-1240 - Istraživanje novih metoda u analitici ljekovitih i bioaktivnih tvari (Nigović, Biljana, MZOS ) ( CroRIS)

Ustanove:
Farmaceutsko-biokemijski fakultet, Zagreb,
Medicinski fakultet, Zagreb,
Klinički bolnički centar Zagreb

Profili:

Avatar Url Biljana Nigović (autor)

Avatar Url Nikša Turk (autor)

Avatar Url Mirko Koršić (autor)

Avatar Url Miranda Sertić (autor)

Avatar Url Ana Mornar Turk (autor)

Citiraj ovu publikaciju:

Mornar, Ana; Sertić, Miranda; Turk, Nikša; Koršić, Mirko; Nigović, Biljana
Determination of mitotane and its metabolites in biological fluids by SPE-HPLC method // Internation Scientific and Professional Conference 13th Ružička Days "Today Science - Tomorrow Industry" / Šubarić, Drago (ur.).
Osijek: Prehrambeno tehnološki fakultet Sveučilišta Josipa Jurja Strossmayera u Osijeku, 2010. (poster, međunarodna recenzija, sažetak, znanstveni)
Mornar, A., Sertić, M., Turk, N., Koršić, M. & Nigović, B. (2010) Determination of mitotane and its metabolites in biological fluids by SPE-HPLC method. U: Šubarić, D. (ur.)Internation Scientific and Professional Conference 13th Ružička Days "Today Science - Tomorrow Industry".
@article{article, author = {Mornar, Ana and Serti\'{c}, Miranda and Turk, Nik\v{s}a and Kor\v{s}i\'{c}, Mirko and Nigovi\'{c}, Biljana}, editor = {\v{S}ubari\'{c}, D.}, year = {2010}, keywords = {mitotane, metabolites, Solid phase extraction, HPLC, biological fluids}, isbn = {978-953-7005-22-1}, title = {Determination of mitotane and its metabolites in biological fluids by SPE-HPLC method}, keyword = {mitotane, metabolites, Solid phase extraction, HPLC, biological fluids}, publisher = {Prehrambeno tehnolo\v{s}ki fakultet Sveu\v{c}ili\v{s}ta Josipa Jurja Strossmayera u Osijeku}, publisherplace = {Vukovar, Hrvatska} }
@article{article, author = {Mornar, Ana and Serti\'{c}, Miranda and Turk, Nik\v{s}a and Kor\v{s}i\'{c}, Mirko and Nigovi\'{c}, Biljana}, editor = {\v{S}ubari\'{c}, D.}, year = {2010}, keywords = {mitotane, metabolites, Solid phase extraction, HPLC, biological fluids}, isbn = {978-953-7005-22-1}, title = {Determination of mitotane and its metabolites in biological fluids by SPE-HPLC method}, keyword = {mitotane, metabolites, Solid phase extraction, HPLC, biological fluids}, publisher = {Prehrambeno tehnolo\v{s}ki fakultet Sveu\v{c}ili\v{s}ta Josipa Jurja Strossmayera u Osijeku}, publisherplace = {Vukovar, Hrvatska} }

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