Naslov
Polymorphism 10034C>T in fibrinogen gamma (FGG) gene, total fibrinogen level and risk of coronary artery disease: the Croatian study

Autori
Bronić, Ana ; Ferenčak, Goran ; Bernat, Robert ; Dumić-Belamarić, Jerka ; Dabelić, Sanja

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
10th Congress of the Croatian Society of Biochemistry and Molecular Biology with international participation: The Secret Life of Biomolecules - book of abstracts / Kovarik, Zrinka ; Varljen, Jadranka - Rijeka : Hrvatsko Društvo za Biotehnologiju, 2010, P20-P20

Skup
10th Congress of the Croatian Society of Biochemistry and Molecular Biology with international participation: The Secret Life of Biomolecules

Mjesto i datum
Opatija, Hrvatska, 15.09.2010. - 18.09.2010

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
polymorphism; fibrinogen gamma chain; coronary artery disease

Sažetak
Coronary artery disease (CAD), the most common type of heart disease, is a leading cause of death and disability throughout the world. Two key events during CAD pathogenesis are atherosclerosis and thrombosis. Recently, a single nucleotide polymorphism (SNP) characterized by a C to T substitution at nucleotide 10034 of the fibrinogen  gene (FGG 10034C>T) has been proposed as a novel risk factor for thrombosis. The aim of this study was to investigate the potential association between FGG 10034C>T polymorphism, total fibrinogen level and risk of CAD. The analysis was performed on subjects who had undergone coronary angiography for investigation of chest pain and suspected CAD in Magdalena Special Hospital for Cardiology in Croatia – overall 114 subjects had angiographically proven CAD (CAD+), while in 68 subjects CAD was angiographically excluded (CAD-). CAD+ patients had >50% stenosis in at least one of the major coronary vessels. FGG genotypes were determined by restriction fragment length polymorphism of the PCR-amplified fragments of DNA isolated from whole blood while fibrinogen levels were measured in plasma. Overall, CC genotype was recorded in 80.2% of subjects (78.9%CAD+ vs. 82.4%CAD-), CT in 18.7% (19.3%CAD+ vs. 17.3% CAD-), and TT in 1.1% (only in 1.8% CAD+) of subjects. The frequency of the rare T allele was 10.4%. There was no significant difference between two groups of patients regarding genotype or allele frequency (p>0.05). Additionally, total fibrinogen levels showed no correlation to patients’ genotype. According to obtained results there is no association between FGG 10034C>T polymorphism and fibrinogen level as well as FGG 10034C>T polymorphism and CAD, although comprehensive case-control study is needed to confirm these preliminary results.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti

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