Pregled bibliografske jedinice broj: 48057
Electrophoretic protein patterns of sugar beet tissue lines
Electrophoretic protein patterns of sugar beet tissue lines // Phyton, 41 (2001), 1; 13-20 (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 48057 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Electrophoretic protein patterns of sugar beet tissue lines
Autori
Krsnik-Rasol, Marijana ; Čipčić, Hana ; Poljuha, Danijela ; Hagege, Daniel
Izvornik
Phyton (0079-2047) 41
(2001), 1;
13-20
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
Beta vulgaris L.; Chewnopodiaceae; differentiation; extracellular glycoproteins; habituation; protein pattern; SDS-PAGE
Sažetak
Cellular and extracellular proteins accumulating in normal (hormone dependent), habituated and crown gall tumour callus lines of sugar beet (Beta vulgaris L. altissima) were studied. Proteins were separated by SDS-polyacrylamide gel electrophoresis and silver stained. Glycoproteins with D-manose in their glycan component were detected on protein blots by concanavalin A-peroxidase staining. A few tissue specific cellular proteins (16, 17, 18, 19, 24, 37 kDa) were detected. Patterns of proteins secreted in the nutrient medium of suspension cultured cells were distinct for each tissue line. Nonorganogenic habituated tissue contained more protein bands in the range between 25 and 66 kDa than the other lines. Among glycosilated extracellular proteins, the 68 kDa protein was common for all tissues, whereas the 45 kDa was distinct for both habituated lines. The nonorganogenic habituated tissue was deficient in the 34 kDa polypeptide, and the crown gall tumour in the 23 kDa one.
Izvorni jezik
Engleski
Znanstvena područja
Biologija
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
Uključenost u ostale bibliografske baze podataka::
- Biological Abstracts
