Naslov
Antioxidant activities and polyphenolic contents of three selected Micromeria species growing in Croatia

Autori
Vladimir-Knežević, Sanda ; Blažeković, Biljana ; Bival Štefan, Maja ; Alegro, Antun

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Planta Medica / Melzig, Matthias F. ; Kolodziej, Herbert - Stuttgart : Georg Thieme Verlag, 2010, 1256-1256

Skup
58th International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research

Mjesto i datum
Berlin, Njemačka, 29.08.2010. - 02.09.2010

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Micromeria croatica; Micromeria juliana; Micromeria thymifolia; antioxidant activity; polyphenols

Sažetak
The genus Micromeria (Lamiaceae) is represented by nine species in Croatia, three of which (M. croatica (Pers.) Schott, M. dalmatica Benth. and M. pseudocroatica Šilic) are endemic [1]. Micromeria species are perennial herbs or dwarf shrubs growing mostly in Mediterranean region. Some of them are traditionally used against heart disorders, headache, colds, wounds and skin infections, as well as condiments [2]. In the present paper, antioxidant activities of M. croatica, M. juliana and M. thymifolia were evaluated using five in vitro antioxidant assays, in comparison with plant polyphenolic constituents and reference antioxidants. All studied plant extracts exhibited considerable activity to scavenge DPPH and hydroxyl free radicals, reducing power, iron-chelating ability and total antioxidant capacity in order: M. croatica > M. juliana > M. thymifolia. Among the tested plants, ethanolic extract of M. croatica was found to be the most effective DPPH radical scavenger (IC50 4.7 μg/ml), even stronger than BHT (IC50 6.5 μg/ml). The highest activity toward hydroxyl free radicals was recorded for the same extract (IC50 249.65 μg/ml). Additionally, it also showed the strongest reducing power (IC50 9.64 μg/ml) and iron-chelating ability (IC50 227.47 μg/ml). In phosphomolybdenum assay, M. croatica displayed twofold lower total antioxidant capacity than ascorbic acid. Total polyphenol (9.69-13.66%), phenolic acid (5.26-6.84%), flavonoid (0.01-0.09%) and tannin contents (3.07-6.48%) in dried plant samples were spectrophotometrically determined. A good correlation between antioxidant activities and contents of phenolic acids and tannins was established, indicating their responsibility for antioxidant capabilities of Micromeria species. References: 1. Lovašen-Eberhardt, Ž. (2000) Nat. Croat. 9:19-20. 2. Duru, M.E. (2004) J. Ethnopharmacol. 94:43-48.

Izvorni jezik
Engleski

Znanstvena područja
Farmacija

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