Naslov
Short-term salt and osmotic stress in Mamillaria gracillis Pfeiff. tissue culture

Autori
Balen, Biljana ; Rogić, Tea ; Šimac, Matija ; Peharec, Petra ; Bar-Zvi, Dudy ; Krsnik-Rasol, Marijana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
FESPB 2010 Book of Abstracts / Beltran Maria, Jose Pio ; Rodriguez, Dolores - Valencia : FESPB, 2010, P01-033

Skup
FESPB 2010 XVII Congress of the Federation of European Societies of Plant Biology

Mjesto i datum
Valencia, Španjolska, 04.07.2010. - 09.07.2010

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
salt stress; osmotic stress; cactus; plant tissue culture

Sažetak
In vitro propagated M. gracilis plants develop calli without any exogenous growth regulators. This habituated calli spontaneously regenerate morphologically normal and hyperhydric shoots. Since the habituation and hyperhydricity are both part of a neoplastic progression, cactus cells were transformed with A. tumefaciens strain B6S3. Tumor line, which was established, never expressed any morphogenic capacity. The aim of this study was to investigate the effect of short-term salt and osmotic stress on the M. gracilis callus and tumor culture. Tissue explants were grown in a liquid nutrient and exposed for 15 min and 3 h to growth medium supplemented with 250 mM NaCl or 500 mM mannitol. Following treatments, tissue was collected and protein extracts were prepared. They were examined with regard to abundance and phosphorylation of ASR1 protein by western blotting and Pro Q Diamond fluorescent dye, respectively. Glycosylation pattern was examined by Pro Q Emerald fluorescent dye and lectin assay. ASR1 was present in both cactus tissues with three isoforms of approximately 42, 26 and 17 kDa. No difference in abundance or phosphorylation of ASR1 in callus and tumor was observed after either 15 min or 3 h treatment with salt. More intensive phosphorylation signal of ASR1 was noticed in callus grown on medium with mannitol for 3 h. Analysis of glycosylation pattern with lectins Con A and GNA indicated stronger protein glycosylation in callus exposed to mannitol after 15 min and 3 h.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA