Naslov
The regeneration ability in common oak (Quercus robur L.) callus cultures

Autori
Pevalek-Kozlina, Branka ; Jelaska, Sibila

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of 1st Slovenian Symposium on Plant Physiology / - Ljubljana, 1993, 21-21

Skup
1st Slovenian Symposium on Plant Physiology

Mjesto i datum
Gozd Martuljek, Slovenija, 29.09.1993. - 01.10.1993

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
common oak; Quercus robur; callus; shoot regeneration

Sažetak
Regeneration of shoots in stem callus cultures of the common oak (Quercus robur L.) was investigated. Internodal segments from in vitro propagated shoots (on GD medium supplemented with 3% sucrose, 0.7% Difco Bacto-agar and 0.9 µM BA) were subcultured on GD medium with different concentrations of BA (0.1, 0.5, 1.0 and 10.0 µM). After four weeks in culture two types of callus regenerated depending on the medium composition: a friable, light brownish coloured (0.1, 10.0 µM BA), and acompact nodular and brown (0.5, 1.0 µM BA). Established callus cultures were attempted by subculturing on GD medium with 0.9 µM BA. Calli initiated on media with 0.1, 1.0 and 10.0 µM BA died, but tissues initiated on medium with 0.5 µM BA regenerated one to six shoots per callus. Regeneration was achieved in 68 to 97% cultures, and was genotype dependent. Shoots were morphologically normal and could be microcloned on medium with 0.9 µM BA.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA