Naslov
Culture conditions and genome instability in callus of Vicia faba

Autori
Papeš, Dražena ; Jelaska, Sibila ; Pevalek, Branka ; Devide, Zvonimir

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
Abstracts of 4th John Innes Symposium the Plant Genome and 2nd International Haploid Conference / - Norwich, 1979, 128-128

Skup
4th John Innes Symposium the Plant Genome and 2nd International Haploid Conference

Mjesto i datum
Norwich, Ujedinjeno Kraljevstvo, 21.08.1979. - 26.08.1979

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
callus culture; Vicia faba; genome instability

Sažetak
The dependence of genome instability on various nutrient media as well as on the condition of subculturing was studied in three strains of Vicia faba callus culture. Growth rate of maintained callus cultures (strains VFS1, VFZ6 and VFT2) were high on the 3 following media: 1. RM-1962, 3% sucrose, 0.2 mgl-1 2, 4-D and 0.2 mgl-1 kinetin ; 2. RM-1962, 3% sucrose, 0.3 mgl-1 2, 4-D ; 3. B5 modified medium (mineral salts double amount without NH4NO3), 3% sucrose, 0.5 mgl-1 2, 4-D and 5.0 mgl-1 kinetin. Karyological analyses of the three strains showed that in young as well in in old subcultures there were diversity of ploidy levels and/or aneuploidy. The beginning subcultures of the strains VFS1 and VFZ6 (obtained from immmature embryos of unripe seeds) showed, however, a larger variability of polyploidy than the beginning subcultures from the strain VFT2 (obtained from mature embryo of ripe seeds). The increase of kinetin concentrations reduced the frequency of polyploids in favour of diploids (hypo- and hyperdiploids respectively). In addition to the instability of chromosome numbers structural changes in chromosomes, especially translocations and inverzions have been observed by the Giemsa C-banding technique. The predominant chromosome numbers were 14-18 with 3Mchromosomes.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA