Naslov
Immunoprotective properties of peptidoglycan monomer linked with zinc in cholestatic jaundice

Autori
Ravlić-Gulan, Jagoda ; Radošević-Stašić, Biserka ; Gulan, Gordan ; Štimac, Davor ; Pavelić, Krešimir ; Rukavina, Daniel

Izvornik
International Archives of Allergy and Immunology (1018-2438) 123 (2000), 4; 354-364

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
peptidoglycan monomer linked with zinc; common bile duct ligation; jaundice; blastogenesis

Sažetak
Background. Previously it was shown that new immunostimulator, peptidoglycan-monomer linked with zinc (PGM-Zn), might have immunocorrective and hepatotropic effects. Owing to this in the present study we investigated its effects on jaundice-induced immunodysfunction, which might be responsible for serious peri and postoperative complications in biliary obstruction. Methods. In vivo effects of PGM-Zn were analysed in mice subjected to common bile duct ligation (CBDL), where we estimated phenotypic profile and cell cycle of thymocytes, splenocytes and phagocytic function of peritoneal macrophages. In vitro effects of PGM-Zn were evaluated on blastogenesis of human peripheral blood mononuclear cells (PBMNC), obtained from healthy donors and stimulated with anti-CD3 monoclonal antibody and/or PMA, in the presence or absence of jaundice serum, obtained from patients with biliary calculosis. Results: Jaundice induced marked disarrangement of lymphatic homeostasis, which at several points might be blocked by PGM-Zn. In mice it delayed the CBDL-induced decline of CD4+CD8+ thymocytes, decreased the proportion of CD8+ T cells, and increased the percentage of CD4-CD8- thymocytes, augmenting simultaneously the proportion of thymic cells in S and G2+M phase of cycle. Similar hyperplastic reaction with increased percentage of CD4+, Ig+ and CD5+ cells was noticed in the spleen, together with the enhanced phagocytic ability of peritoneal macrophages. In human PBMNC jaundice reduced the percentages of CD3, CD5, CD4, CD8, and HLA-DR expressing cells and increased the proportion of CD25 and perforin positive lymphocytes. PGM-Zn given in vitro was able to abrogate the antiproliferative activity of jaundice serum on PMA and anti-CD3+PMA induced blastogenesis.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA