Naslov
Nitric oxide (NO) production in patiens with urinary tract infections and various renal diseases

Autori
Morović-Vergles, Jadranka ; Čulo, Filip

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Immunology Letters / Wagner, H ; Heeg, K ; Pfeffer, K. - Amsterdam : Elsevier, 1997, 440-440

Skup
13th European Immunology Meeting

Mjesto i datum
Amsterdam, Nizozemska, 22.06.1997. - 25.06.1997

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
NO; renal diseases

Sažetak
Introduction: Nitric oxide (NO) is important defense molecule secreted by macrophages when encountered by bacteria, intracellular parasites and tumor cells. Various stimuli (BCG, LPS, interferon-g, TNF-a, IL-1) induce secretion of NO by mouse macrophages and endothelial cells. However, its secretion under similar conditions by human macrophages is not so well proven. In this study we analyzed the production of NO in uropoietic system in patients with urinary tract infections, as well as in patients with various renal diseases. Materials and Methods: The study included 157 patients hospitalized at nephrologic department and 15 healthy volunteers. Eighty three patients had bacterial urinary tract infections (UTI) and the rest (74) had various renal diseases. The production of NO was determined by measuring nitrite (NO2-) concentration in urine. In parallel, the concentration of nitrates (NO3-) was also measured. The concentration of nitrite was determined by a colorimetric method, using Griess reagent. One hundred ml of urine was mixed with the same volume of Griess reagent in 96-well plates and the absorbance was in microplate optical reader at 550 nm. Standard curve for NO was obtained by using sodium nitrite as a standard. Nitrate concentration was determined indirectly by their reduction with elementary zinc into nitrite. Standard laboratory examinations of urine and blood was performed in all patients. Results: Nitrite (NO2-) could not be detected in urine of 15 healthy volunteers. However, nitrite could be detected in 54 out of 157 (34.4%) patients with renal diseases. Out of 83 patients with UTI, NO was detected in 34 patients (41%). In most patients with UTI the causative agent was E. coli (39 patients). NO could be detected in 18 or 46% of the latter patients. NO was positive in urine in variable proportions of patients with different renal disease, including hypoplastic kidneys, lypus nephritis etc. Nitrates were found in significant amounts in urine of all patients and healthy controls, and its concentration did not differ significantly between them or between different groups of patients There was not significant correlation between level of nitrites or nitrates in urine and ESR, number of leukocytes or erythrocytes, level of proteinuria and blood pressure. Conclusion: NO could be detected in urine of about one third of patients hospitalized at nephrologic department. Most of them have urinary tract infection. However, only 41% of patients with UTI have detectable NO in urine. About one fourth of patients with various renal diseases have positive nitrite in urine. Nitrate concentrations in urine did not differ between healthy subjects and patients with UTI or various renal diseases.

Izvorni jezik
Engleski

Znanstvena područja
Farmacija

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