Naslov
Microclonal multiplication of wild cherry (Prunus avium L.) from shoot tips and root sucker buds

Autori
Pevalek-Kozlina, Branka ; Michler, Charles H. ; Jelaska, Sibila

Izvornik
Acta botanica Croatica (0365-0588) 53 (1994); 31-38

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
micropropagation; wild cherry; Prunus avium

Sažetak
The effects of different combinations and concentrations of the growth regulators: 6-benzylaminopurine (BA), 6-furfurylaminopurine (KIN), N6-(2-isopentenyl) adenine (2iP), indole-3-butyric acid (IBA), indole-3-acetic acid (IAA) and α-naphthaleneacetic acid (NAA) on axillary shoot multiplication rates for wild cherry (Prunus avium L.) shoot explants were determined. Apical shoot tips and axillary buds from juvenile trees (5-year old) and from root suckers of mature trees (55-year old) were used as initial explants for establishment of multiple shoot cultures. Factorial tests for growth regulator interactions with the two genotypes showed significant differences in shoot multiplication rate with numerous auxin- and cytokinin-amended basal medium. For genotype P5, the most effective growth regulator combination was 5.0 mg/L KIN and 1.0 mg/L IAA. Genotype 5/11 responded best to 1.0 mg/L BA and 5.0 mg/L IBA. Both clones multiplied easely in culture, and shoot multiplication of mature genotype was not inhibited.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA