Pregled bibliografske jedinice broj: 476301
On Relevance of Codon Usage to Expression of Synthetic and Natural Genes in Escherichia coli
On Relevance of Codon Usage to Expression of Synthetic and Natural Genes in Escherichia coli // Genetics, 185 (2010), 1129-1134 doi:10.1534/genetics.110.115477 (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 476301 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
On Relevance of Codon Usage to Expression of Synthetic and Natural Genes in Escherichia coli
Autori
Supek, Fran ; Šmuc, Tomislav
Izvornik
Genetics (0016-6731) 185
(2010);
1129-1134
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
codon bias; mRNA secondary structure; protein translation; heterologous expression; Escherichia coli
Sažetak
A recent investigation concluded that codon bias did not affect expression of green fluorescent protein (GFP) variants in Escherichia coli, while stability of an mRNA secondary structure near the 5' end played a dominant role. We demonstrate that combining the two variables using regression trees or support vector regression yields a biologically plausible model with better support in the GFP dataset and in other experimental data: codon usage is relevant for protein levels if the 5' mRNA structures are not strong. Natural E. coli genes had weaker 5' mRNA structures than the examined set of GFP variants, and did not exhibit a correlation between the folding free energy of 5' mRNA structures and protein expression.
Izvorni jezik
Engleski
Znanstvena područja
Biologija, Računarstvo, Biotehnologija
POVEZANOST RADA
Projekti:
00980501
098-0000000-3168 - Strojno učenje prediktivnih modela u računalnoj biologiji (Šmuc, Tomislav, MZOS ) ( CroRIS)
Ustanove:
Institut "Ruđer Bošković", Zagreb
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE
