Naslov
Antigen doses used for challenge in mice model of asthma differently affect airway hyperresponsiveness and airway inflammation in the lungs

Autori
Bošnjak, Berislav ; Ivetić Tkalčević, Vanesa ; Perović, Daniela ; Ferenčić, Željko ; Dominis Kramarić, Miroslava ; Marković, Stribor ; Čulić, Ognjen ; Eraković, Vesna ; Hrvačić, Boška

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of abstracts of the 2005 Annual Meeting of the Croatian Immunological Society / - , 2005

Skup
Annual Meeting of the Croatian Immunological Society

Mjesto i datum
Božava, Hrvatska, 29.09.2005. - 02.10.2005

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
asthma; inflammation; hyperresponsiveness; ovalbumin; microarray; MCP-1; Spp1

Sažetak
Airway inflammation (AI) and hyperresponsiveness (AHR) are two asthma hallmarks. However, the function of particular cell types, i.e. eosinophils or T-cells, in the development of AHR is still not unambigously elucidated. We hypothesized that challenge of mice with varying antigen doses could provide new insights into the complex mechanisms by which AI enhances AHR. AHR was detected only in mice challenged with larger OVA dose, indicating dependence of AHR on the antigen dose used for challenge. In contrast to AHR, accumulation of inflammatory cells was not influenced by applied antigen dose. Subsequent DNA microarray analysis revealed that larger OVA dose induces more prominent expression of macrophage chemoattractant protein 1 (MCP-1) and secreted phosphoprotein 1 (Spp1). Our results imply that AHR is more sensitive to effects of antigen dose than accumulation of inflammatory cells.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA