Naslov
Biocatalytical hexanol oxidation

Autori
Matun, Vlatka ; Vasić-Rački, Đurđa ; Vrsalović Presečki, Ana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Applied Biocatalysis, 6th meeting of students and university professors, Book of Abstracts / Vasić-Rački, Đurđa ; Vrsalović Presečki, Ana - Zagreb : Pasanec d.o.o., 2010, 22-22

ISBN
978-553-6470-50-1

Skup
Applied Biocatalysis, 6th meeting of students and university professors

Mjesto i datum
Zagreb, Hrvatska, 09.06.2010

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
baker's yeast; biocatalyst; two-phase system; hexanol oxidation

Sažetak
Biotransformations can be performed by using either whole cells or isolated enzymes. Compared with isolated enzymes, whole cell catalysts can be much more readily and inexpensively prepared [1]. Yeasts are source of number of enzymes and are consider as the most popular biocatalysts in a form of whole cell. Hexanol oxidation catalyzed by whole cells of baker's yeast has been performed in this work. The reaction is catalyzed by the enzyme alcohol dehydrogenase (ADH) that is in yeast cell responsible for the reaction of the acetaldehyde reduction. Enzyme alcohol dehydrogenase belongs to oxidoreductase which means that for its action requires a presence of coenzyme. For the biocatalytical hexanol oxidation beside enzyme the coenzyme NAD+ is necessary. Considering the high price of coenzyme its regeneration has been performed by the addition of oxygen that allows the respiratory chain of yeast cell to provide oxidized cofactor. The reaction of hexanol oxidation was performed by permeabilized and non-permeabilized cells. Permeabilization has been performed by treating the yeast cell with detergent CTAB to remove the barrier for the faster diffusion of substrate/ product across the cell membrane. Hexanal and hexanoic acid can be detected as the products of biocatalytical hexanol oxidation catalyzed by baker's yeast. According to the literature the production of hexanoic acid could be obviate by carrying out the oxidation in the biphasic system [2]. Considering that in this work the hexanol oxidation has been performed in the water and biphasic (hexane-water) medium. In the water medium 100 % hexanol conversion was achieved by permeabilized and non-permeabilized cells. Hexanol conversion in the biphasic medium using non-permeabilized cells was cca 40 %, while with permeabilized cells it was around 50 %. In all cases the yield on hexanal was extremely low, and hexanoic acid was the main product of the reaction. [1] Ishige T., Honda K., Shimizu S., Curr Opin Chem Biol. 2005, 9:174-180. [2] Capparet L., Larroche C., Biocatal Biotransform, 2004, 22:291-296.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

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