Naslov
Double layer-charge displacement by adhesion and spreading of living cells

Autori
Žutić, Vera ; Svetličić, Vesna ; Ivošević, Nadica ; Kovač, Solveg

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstract of the second international symposium on contact angle, wettability and adhesion / Mittal, K.L. Lacombe, R.H. - Newark (NJ), 2000

Skup
Second international symposium on contact angle, wettability and adhesion

Mjesto i datum
Newark (NJ), Sjedinjene Američke Države, 21.06.2000. - 23.06.2000

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
charge displacement; adhesion sensor; spreading; living cells; Dunaliella tertiolecta

Sažetak
The potentiostatic control of surface charge density and interfacial tension of an electrode aqueous interface offers a possibility for direct studies of non-specific interactions in cell adhesion and development of adhesion based sensors for environmental particles. Unicellular marine alga, Dunaliella tertiolecta (Chlorophyceae) of micrometer size and flexible cell membrane was used as a model and 0.1 M NaCl, pH=8, as supporting electrolyte. The dropping mercury electrode acts as an in situ adhesion sensor with adhesion and spreading rates being enhanced by the hydrodynamic regime of electrode's growing fluid interface. The adhesion of single cells causes a displacement of counter-ions from the electrical double layer. The instant flow of compensating current reflects the dynamics of adhesive contact formation and spreading of a cell. The amperometric technique allows precise recording of adhesion signals of individual cells and measurement of cell contact area, the distance of the closest approach and calculation of adhesion forces.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

POVEZANOST RADA