Naslov
Different transforming potential of normal and mutated hepatitis B virus X gene

Autori
Tadić, Zoran

Izvornik
Libri oncologici: Croatian journal of oncology (0300-8142) 21 (1992); 153-160

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Hepatitis B virus; X gene; Transformation

Sažetak
Hepatitis B virus is etiologic agent of acute hepatitis and is considered to be a major factor in hepatocellular carcinoma initiation. Here, the transforming capability of normal and mutated HBV X gene was investigated. NIH 3T3 cellswere transfected with plasmids carrying normal X gene and a frameshift mutant in which onlysmall part is translated correctly. Both constructs were driven by Rous sarcoma virus LTH promoter. The transformed phenotype was tested in focus-forming assay and ability to grow in semisolid medium (soft agar). In both tests mutated HEV X gene showed significantlygreater transforming capability than its normal counterpart. The possible mechanisms of these observations are discussed with the special emphasis on additional open reading frames within the X-ORF.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA