Naslov
Characterization of β-glucans isolated from brewer’s yeast and dried by different methods

Autori
Zechner-Krpan, Vesna ; Petravić-Tominac, Vlatka ; Franulović, Iva ; Sajli, Lana ; Đaković, Senka ; Filipović-Grčić, Jelena

Izvornik
Food technology and biotechnology (1330-9862) 48 (2010), 2; 189-197

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
β-glucan ; β-glucan isolation ; brewer’s yeast ; β-glucan ; β-glucan siolation ; brewer’s yeast ; Saccharomyces cerevisiae
(β-glucan ; β-glucan isolation ; brewer’s yeast ; Saccharomyces cerevisiae)

Sažetak
Two different procedures have been used for isolation of water-insoluble β-glucans from brewer’s yeast: alkaline-acidic isolation (AA) and alkaline-acidic isolation with mannoprotein removal (AAM). The obtained β-glucans were then dried by air-drying, lyophilization and combination of sonication and spray-drying. β-Glucan preparations obtained by AA and AAM isolations had similar values of dry mass, total polysaccharides, proteins and organic elemental microanalysis. The mass fractions of β-glucan in total polysaccharides were significantly affected by different isolation procedures. Fourier transform infra-red (FTIR) spectra of all preparations had the appearance typical for (1-3)-β-D-glucan. Lyophilization and especially air-drying caused a higher degree of agglomeration and changes in β-glucan microstructure. Sonication followed by spray-drying resulted in minimal structural changes and negligible formation of agglomerates.

Izvorni jezik
Engleski

Znanstvena područja
Farmacija, Biotehnologija

POVEZANOST RADA