Naslov
Selective hydrolyses catalyzed by esterases from mammalian sera

Autori
Tomić, Srđanka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts / Vančik, Hrvoj - Varaždin, 2000, 46-47

Skup
Central European Chemical Meeting

Mjesto i datum
Varaždin, Hrvatska, 14.06.2000. - 17.06.2000

Vrsta sudjelovanja
Pozvano predavanje

Vrsta recenzije
Nije recenziran

Ključne riječi
Mammalian sera esterases; regioselctive deacylation of monosaccharides

Sažetak
Several esterases present in mammalian sera were shown to hydrolyze carbohydrate esters in a regioselective and/or chemoselective manner. Esterase activities with specificity for carbohydrate esters were isolated from rabbit and guinea-pig sera. They were characterized by using series of substrates and inhibitors specific for various possible types of esterases usually present in mammalian sera. Kinetic studies were also undertaken with radiolabelled monosaccharides as substrates. Separated enzyme fractions were then applied in regio and chemoselective deacylations of acylated monosaccharides. Acyls used were acetyls, pivaloyls and N-acylaminoacyls. The degree of regioselectivity and the direction of hydrolysis depend on the sugar structure, enzyme source, and the particular characteristics of each isolated fraction. Furthermore, high degree of chemoselectivity was observed in the case of compounds bearing structurally different acyl groups. The possibility of using these enzymes in deesterifications of other classes of biologically active compounds was explored as well. Thus, it was found that esters derived from quiniclidin-3-ol can be subjected to enzymic hydrolysis catalyzed by esterases from mammalian sera as well as by some commercially available esterases. Since the synthesis of these esters produces racemates the possibility of their resolution by using the same enzymes is being examined as well.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

POVEZANOST RADA