Naslov
Electroconvulsive shock-induced seizures in rats : the influence of ethanol on the brain free fatty acid level and GPX activity

Autori
Varljen, Jadranka ; Eraković, Vesna ; Župan, Gordana ; Simonić, Ante

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of the XXXVII. European Congress of Toxicology : EUROTOX '99 ; u: Toxicology Letters. Supplement 109 (1999) (S1) / - , 1999, 81-81

Skup
European Congress of Toxicology (37 ; 1999)

Mjesto i datum
Oslo, Norveška, 27.06.1999. - 30.06.1999

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Sažetak
Enhanced neuronal activity during epileptic discharge causes a massive influx of extracellular calcium ions. The elevated intracellular concentration of Ca2+ triggers a variety of biochemical processes including the activation of membrane phospholipases and the liberation of free fatty acids (FFA) diacylglycerols, eicosanoids, lipid peroxides and free radicals. The aim of this study was to determine the influence of ethanol, common substance of abuse and usual component of vehicle solutions, on the brain FFA level and glutathion peroxidase (GPX) activity in rats with electtnconvulsive (ECS) shock induced seizures. Experiments were carried out on Hannover-Wistar rats. Prior to single or repeated ECS treatment (five ECSs over five day-period with regular 24 h recovery period between them) the animals of control group did not receive any substance, while the others received i.p. 1 ml/kg of ethanol and propylene glycol 400 ( 1:1 V/V) mixture, a common vehicle for calcium channel blockers. This way rats received 0.4 g/kg ethanol. All rats were decapitated 3 min after single ECS and 24 h after the last ECS in multiple ECS treatment. The brains were quickly removed and frozen in liquid nitrogen. The FFAs were quantified using gas chromatography. GPX activity was determined using a method according to Paglia and Valentine. The results indicate that ECS-induced seizures cause the brain FFA accumulation. Administration of ethanol and propylene glycol mixture induced an increase of the brain free oleic, arachidonic and docosahexaenoic acid level. ECS, single or repeated, induced a decrease of GPX activity in various brain regions. Administration of tested mixture induced an increase of the GPX activity in the rat cerebral cortex, cerebellum, hippocampus and pons-medula region.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA