Naslov
Point mutation of tumor suppressor gene p53 in two highly malignant metastatic insulinomas

Autori
Pavelić, Krešimir ; Hrašćan, Reno ; Čabrijan, Tomislav ; Križanac, Šimun ; Eljuga, Damir ; Pavelić, Ljubomir ; Karapandža, Nikola ; Kusić, Zvonimir ; Spaventi, Šime ; Pavelić, Jasminka

Izvornik
Tumor Diagnostik und Therapie (0722-219X) 18 (1997), 4; 102-106

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
malignant insulinoma; point mutations; p53 gene

Sažetak
Background: The objective of the study was to define the molecular alterations of the tumor suppressor p53 gene that are associated with tumors of the endocrine pancreas: benign and malignant pancreatic insulinomas. Materials and Methods: The expression of the mutant p53 protein was evaluated immunohistochemically on formalin-fixed paraffin-embedded tumor sections (29 benign insulinoma tissues, 9 malignant insulinomas, 2 B-cell hyperplasia specimens, 3 normal pancreatic tissue samples) using monoclonal (Ab-6) antibodies for p53 proteins. Point mutations of the p53 gene were defined by direct sequencing of amplified DNA (asymmetric PCR, exons 4-9) obtained from one B-cell hyperplasia tissue, 5 benign insulinomas and 5 malignant insulinomas. Results: All of the normal pancreatic and B-cell hyperplasia samples as well as most of the benign insulinoma tissues (24 out of 29 samples) were negative for the mutated p53 protein. In malignant insulinomas an overexpression of the p53 protein was found (in 6 out of 9 samples). Point mutation (single base substitution) was detected in two out of five malignant insulinomas. The first tumor harbored a point mutation at codon 151 (CCC->TCC), while the second tumor harbored a mutation at codon 248 (CGG->GGG). Both patients survived 6 and 8 months respectively, after surgery and chemotherapy. No p53 mutations were identified in 5 benign insulinomas and one hyperplasia of the endocrine pancreas. Conclusions: Our results suggest a link between p53 gene alterations and the malignant stage of pancreatic insulinomas. Based upon these results, we have concluded that, in some rare cases, point mutation of the p53 tumor-suppressor gene may play a role in the development of malignant metastatic insulinomas. ŠReferences: 16Ć

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti

POVEZANOST RADA