Naslov
Kapilarna elektroforeza u farmaciji
(Capillary electrophoresis in pharmacy)

Autori
Damić, Miranda ; Nigović, Biljana

Izvornik
Farmaceutski glasnik : glasilo Hrvatskog farmaceutskog društva (0014-8202) 66 (2010), 4; 195-207

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, pregledni rad, stručni

Ključne riječi
Kapilarna elektroforeza; Analitika lijekova; Farmacija
(Capillary electrophoresis; Pharmaceutical Analysis; Pharmacy)

Sažetak
Capillary electrophoresis is a new separation technique. It is an analytical technique that usually complements or replaces high performance liquid chromatography. Basic principle of the technique is the migration of charged species under applied electric field towards one of the electrodes through a narrow capillary filled with electrolyte solution. Main advantages over other chromatographic methods are short analysis time, high efficiency, small sample and solvent volumes, low costs, simplicity and ecological acceptability. Capillary electrophoresis has several modes of separation which allows analysis of different types of analytes. Capillary electrophoresis has found its application in the analysis of large molecules like proteins and nucleic acids, small organic molecules such as drugs, hormones, plant metabolites, food product ingredients, small inorganic ions etc. It is a method of choice when rapid results are desired. Capillary electrophoresis has become the predominant technique for the analysis of both basic and chiral pharmaceuticals, it is leaving its mark in biotechnology and promises to be a valuable tool in troubleshooting in proteome-wide analysis, DNA sequencing and genotyping. Despites all advantages and application possibilities, capillary electrophoresis is still not enough explored and used in routine analysis. A review of different capillary electrophoresis techniques principles and applications in pharmacy is presented in this article.

Izvorni jezik
Hrvatski

Znanstvena područja
Farmacija

POVEZANOST RADA