Naslov
Chemical Composition and Antimicrobial Activity of Volatiles from Degenia velebitica, European Stenoendemic Plant of the Brassicaceae Family
(Chemical Composition and Antimicrobial Activity of Volatiles from Degenia velebitica, European Stenoendemic Plant of Brassicaceae Family)

Autori
Mastelić, Josip ; Blažević, Ivica ; Kosalec, Ivan

Izvornik
Chemistry & biodiversity (1612-1872) 7 (2010), 11; 2755-2765

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Degenia velebitica; Brassicaceae; glucosinolates; O-glycosidically bound volatiles; hydrodistillation-adsorption method (HDA); antimicrobial activity

Sažetak
Free and glucosidic bound leaf volatiles of Degenia velebitica were isolated and fractionated simultaneously into water soluble, non-soluble and high volatile compounds by hydrodistillation-adsorption (HDA) method and analysed by gas chromatography – mass spectrometry. In general, among twenty four identified, the main compounds obtained by HDA method were sulphur and/or nitrogen containing compounds. They were 6-(methylthio)hexanenitrile (26.78%), dimethyl trisulphide (26.35%), 3, 4, 5-trimethylpyrazole (13.33%), 5-hexenenitrile (10.11%), dimethyl tetrasulphide (4.93%) and 4-pentenyl isothiocyanate (4.45%). In addition, O-glycosidically bound volatiles and free volatiles were isolated by solvent extraction. Sixteen volatile O-aglycones and twelve free volatile components were identified. The main O-aglycones were eugenol (24.15%), 4-vinyl-2-methoxyphenol (11.50%), benzyl alcohol (9.49%) and the main free volatile compounds were (Z, Z)-octa-9, 12-dienic acid (38.35%), hexadecanoic acid (22.64%) and phytol (5.80%). Water soluble volatile fraction obtained by HDA method, containing mostly glucosinolate degradation products and 3, 4, 5-trimethylpyrazole, was evaluated for antimicrobial activity using the diffusion method, minimal inhibitory concentrations as well as minimal microbicidal concentrations. Isolate expressed activity against tested Gram-positive, Gram-negative bacterial species and yeast with MIC values equal or lower of 16.7 µg/ml.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

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