Naslov
Is the polymorphism in the serotonin type 1B receptor gene associated with alcohol dependence and aggressive behavior?

Autori
Muck-Šeler, Dorotea ; Mustapić, Maja ; Nenadić-Šviglin, Korona ; Babić, Ana ; Nedić, Gordana ; Nikolac, Matea ; Pivac, Nela

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Program & Abstract Book of the 24th Danube Congress of Psychiatry & 12th Central European Neuropsychopharmacological Symposium / Jakovljević, Miro - Zagreb : Medicinska naklada, 2010, 60-61

Skup
24th Danube Congress of Psychiatry & 12th Central European Neuropsychopharmacological Symposium

Mjesto i datum
Zagreb, Hrvatska, 05.05.2010. - 08.05.2010

Vrsta sudjelovanja
Pozvano predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
genetic variants of the HTR1B gene; aggressive phenotypes; alcohol dependence

Sažetak
Serotonergic receptors type 1B (5HT1B) are G protein-coupled receptors distributed in the central nervous system and in the variety of vascular tissues. The 5HT1B autoreceptors or heteroreceptors are located on the presynaptic and postsynaptic nerve terminals and mediate the release of serotonin and other neurotransmitters like catecholamines and GABA. Animal studies have indicated that the activation of 5-HT1B receptors regulates locomotor activity, while knockout mice lacking the 5HT1B receptor exhibit hyperactivity, aggression and greater alcohol consumption. In humans 5-HT1B receptor is encoded by the gene HTR1B (OMIM*182131) located on chromosome 6 and consists of a single exon, encoding a 390 amino acid peptide. The HTR1B gene has been postulated to play a role in alcohol consumption. It was considered as a candidate gene for alcohol dependence, aggressive behavior and antisocial personality traits but with inconsistent results. A significantly higher frequency of C allele of the G861C polymorphism of HTR1B gene was found in Finnish and German subjects with alcohol dependence and antisocial behavior when compared to allele distribution in healthy relatives and control subjects, but not in comparison to alcohol dependent subjects without antisocial personality features. In contrast, some authors reported higher frequency of the opposite allele G in patients with alcoholism as compared to the allele frequency in healthy controls, although this study included only a small proportion of patients with antisocial characteristics. The aim of the present study was to determine the association between genetic variants of the HTR1B gene and aggressive phenotypes in patients with alcohol dependence. The participants were 264 male subjects, who were admitted to the Centre for Alcoholism and Other Addictions, Psychiatric Hospital Vrapče, due to withdrawal syndrome related to alcohol dependency. Withdrawal symptoms were of non-psychotic type, intensified 1 week before the admission. The Brown–Goodwin Lifetime Aggression Scale was used as a continuous measure of aggression and antisocial behaviors in patients with alcoholism. The HTR1B 3’UTR functional polymorphism (rs13212041 ; A/G) was genotyped using a PCR-based TaqMan allelic discrimination assay by using commercial kits based on the fluorescently-labeled probes (Applied Biosystems, TaqMan). In patients with alcohol dependence the frequency of AA, AG and GG genotypes of the HTR1B gene polymorphism was 62.9%, 32.2% and 4.9% respectively. The A allele was presented in 78.9% of subjects. No differences in the genotype (χ2=0.319 ; df=2 ; p=0.852) and allele (χ2=0.000 ; df=1 ; p=0.996) frequencies were found among patients with alcohol dependence, subdivided according to the Brown–Goodwin Lifetime Aggression Scale in aggressive (scores ≥ 8) and non-aggressive (scores < 8) patients. Although literature data indicate the role of 5-HT1B receptors in alcohol dependence and alcoholism-related aggressive behavior, we have failed to find an association between genetic variants of the HTR1B gene and aggressive behavior in patients with alcohol dependence. It is possible that alcohol-dependent personality traits could be regulated with other important functional polymorphism located within the regulatory area outside the coding region on chromosome 6.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti

POVEZANOST RADA