Naslov
Evaluation of multiplexed fluorescent micropheres immunoassay for detection of autoantibodies to nuclear antigens
(Evaluation of multiplexed fluorescent microspheres immunoassay for detection of autoantibodies to nuclear antigens)

Autori
Škaričić, Ana ; Rnjak, Lana ; Kozmar, Ana ; Rudolf, Marija ; Malenica, Branko

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
European journal of immunology (Suppl. 1) Abstracts 2nd European Congress of Immunology / Livingstone Cate - Weinheim : Wiley-Blackwell, 2009, S3-S808

Skup
2nd European congress of Immunology

Mjesto i datum
Berlin, Njemačka, 13.09.2009. - 16.09.2009

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
ANA; ENA; AtheNA-ANA; systemic autoimmune diseases

Sažetak
Objectives: Anti-nuclear autoantibodies (ANA-ENA) directed against various nuclear autoantigens characterise systemic rheumatic diseases. Some of them asigned in the classification for systemic lupus erythematosus (SLE), mixed connective tissue disease (MTCD), systemic sclerosis (ScS) and Sjogren syndrome (SS). Because ANA testing is a critical part of diagnopsis in systemic rheumatic diseases, it is important to detect them very carefully. The aim of the study was to examine the value of the polystirene microsphere-based fluorescent assay in determinatio of autoantibodies to nuclear autoantigens. Methods: The presence of ANA-ENA autoantibodies and their target autoantigens were detected by indirect immunofluorescence (IIF) on HEp-2 cells, antigen specific enzyme-immunoassay (ELISA)and with microsphere-based fluorescent immunoassay (AtheNA Multi-Lyte ANA test) in 42 patients with sLE and 164 patient suspicious systemic autoimmune diseases. The ANA and autoantibodies to nine different autoantigens (SS-A, SS-B, Sm, RNP, DNA topo I, ds-DNA, CENP B, Jo-1 and Hitone) were tested. The sensitivity and concordance of these tests for detection of ANA-ENA were compare by Kappa test. Results: Comparison of aNA screening results by IIF (HEp-2) with enzyme-immunoassay and with AtheNA Multi-Lyte ANA test showed a high rate of concordance (Kappa value from 0.289-0.793 and agreement from 88-98%). Positive disrepant results were found for ds-DNA specificity in 12.5% (25/200) specimens by AtheNA technology, while all tested sera were negative for this antibody by ELISA. Negative discrepant results were observed by AtheNA system for anti-ds-DNA (1.5% ; 3/200). In 7 serum samples (3.%% ; 7/200) we found completely different target antigens by ELISA (ds-DNA) and by AtheNA technology (SS-A, RNP or SS-A, SS-B). Very good concordance in the detection of individual target autoantigens was observed between ELISA and microsphere-based assay (Kappa value from 0.306-0.782 and agreement from 90-98%). Conclusion: Our results suggest that the AtheNA Multi-Lyte ANA test system may be a useful diagnostic tool for ANA-ENA determination

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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