Naslov
Značakj testa s mikrokuglicama obilježenim fluorescentnim bojama u detekciji autoantitijela protiv nuklearnih antigena (ANA)
(Evaluation of multiplexed fluorescent micropheres immunoassay for detection of autoantibodies to nuclear antigens)

Autori
Kozmar, Ana ; Škaričić, Ana ; Rnjak, Lana ; Rudolf, Marija ; Radić, Nives ; Malenica, Branko

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Biochemia Medica (Suppl 1) Book of abstracts 6th Croatian Congress of Medical Biochemists with international participation / Topić, Elizabeta ; Čvorišćec, Dubravka - Zagreb : Hrvatsko društvo za medicinsku biokemiju i laboratorijsku medicinu (HDMBLM), 2009, S1-S192

Skup
6th Croatian Congress of Medical Biochemists with international participation

Mjesto i datum
Supetar, Hrvatska, 30.09.2009. - 04.10.2009

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
ANA; ENA; AtheNA-ANA; sistemske autoimune bolesti
(ANA; ENA; AtheNA; systemic autoimmune diseases)

Sažetak
Introduction: Anti-nuclear autoantibodies (ANA-ENA) directed against various cell nuclear autoantigens characterize systemic rheumatic diseases. some of them have assigned in the classification for systemic lupus erythematosuis (SLE), mixed connective tissue disease (MCTD), systemic sclerosis (ScS) and sjogren syndrome (SS). Because ANA testing is a critical part of diagnosis in systemic rheumatic diseases, it is important to detect them very carefully. Indirect immunofluorescence (IIF) on HEp-2 cells, ELISA and polysryrene microsphere-based fluorescent assay have been used for this purpose. Materials and Methods. The aim of our study was to compare sensitivity and concordance of these tests for detection of ANA-ENA and determination of nine different autoantigens in 42 patients with SLE and 164 patients with suspicious systemic autoimmune diseases. Results: Comparison of ANA screening results by IIF with ELISA and AtheNA Multi-Lyte ANA test system, showed a high rate of concordance (Kappa value from 0.289-0.793 and agreement from 88-89%). Positive discrepant results were found for ds-DNA specificity in 12.5% (25/200) specimens by AtheNA technology while all tested sera were negative for this antibody by ELISA. Negative disrepant results were observed by AtheNA system for anti-ds-DNA (1.5% ; 3/200). In 7 serum samples (3.5% ; 7/200) we found completely different target antigens by ELISA (ds-DNA) and by AtheNA technology (SS-A, RNP or SS-A, SS-B). Very good concordance in the detection of individual target autoantigens was observed between ELISA and microsphere-based assay (Kappa value from 0.306-0.708 and agreement from 90-98%). Conclusion: Our results suggest that the AtheNA Multi-Lyte ANA test system may be a useful diagnostic tool for ANA-ENA determination

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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