Naslov
Advantages and disadvantages of voltammetric method in studying cadmium-metallothionein interactions

Autori
Erk, Marijana ; Raspor, Biserka

Izvornik
Cellular and Molecular Biology (0145-5680) 46 (2000), 2; 269-281

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Metallothionein; Isoforms; Dithiotreitol; Cadmium binding; Mytilus galloprovincialis; Voltammetry

Sažetak
A sensitive and chemical species-selective technique of differential pulse anodic stripping voltammetry (DPASV) was applied in studying the cadmium-metallothionein (Cd-MT) interaction. The amperometric titrations of the purified MT20 and MT10 fractions, isolated by verified biochemical procedures from the digestive gland of cadmium-exposed mussels Mytilus galloprovincialis, with Cd^2+ ions were performed in the buffered sodium chloride solution of 0.59 M ionic strenght, pH=7.9 and 25°C. Applying the DPASV method at various cadmium to metallothionein ratio several groups of chemical species were recorded. The data on the available ligand concentration to complex cadmium ions (C_L), the apparent concentration stability constants (K^,) of the respective complexes and the reliability of the determined complexing parameters are discussed. In quantifying the Cd-MT interaction the interference of dithiotreitol (DTT), which is used as the reducing agent in isolation and purification of MTs, is documented.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

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