Naslov
Variations and interrelation in quantification of mumps virus by two methods based on cytopathic effect

Autori
Forčić, Dubravko ; Košutić Gulija, Tanja ; Šantak, Maja ; Jug, Renata ; Ivančić Jelecki, Jelena ; Markušić, Maja ; Mažuran, Renata

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
European Congress of Virology (4 ; 2010)

Mjesto i datum
Cernobbio, Italija, 07.04.2010. - 11.04.2010

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
mumps; CPE; plaque

Sažetak
Conventional assays in the virological laboratories for identification of a virus isolate and quantification of infectious virus particles are dependent on the observation of cytopathic effect (CPE). Like in many virus samples the quantification of infectious mumps virus particles is routinely performed by plaque forming unit (PFU) assay and/or 50% cell culture infective dose (CCID50) assay. In this study we analyze variations and interrelation in quantification of nine mumps virus strains by these two methods using standard protocols. Strong intrinsic characteristic of each mumps virus strain to interact distinctively with Vero cell culture results in a unique CPE. Thus the titer values of analyzed mumps virus strains arised from the two methods differed significantly for some strains while were almost identical for others. The results reported here indicate that care should be taken when comparing the numbers of infectious virus particles in virus samples obtained by these methods of quantification.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Biotehnologija

POVEZANOST RADA