Naslov
Influence of different growth factors on rat embryo differentiation in vitro

Autori
Belovari, Tatjana ; Marić, Svjetlana ; Čošić, Maja ; Bijelić, Nikola ; Krčmar, Stjepan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Life Sciences / Pabst M A, Zellnig G - Graz : Verlag der TU Graz, 2009, 281-282

Skup
Multinational Congress on Microscopy

Mjesto i datum
Graz, Austrija, 30.08.2009. - 04.09.2009

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
in vitro; rat embryo; differentiation
(n vitro; rat embryo; differentiation)

Sažetak
Cultivation of rat embryos in vitro is the one of the experimental systems for studying developmental processes in mammals. Cultivation in chemically defined medium in organ culture enables investigation of effects of different substances on growth and differentiation of mid-gastrula embryos (1-4). In this study, effects of fibroblast growth factor (FGF), epidermal growth factor (EGF) and transferrin on growth and differentiation were tested. Rat embryos (E 9.5) were cultivated during 14 days in chemically defined Eagle's Minimal Essential Medium (MEM) alone, in MEM with FGF, in MEM with EGF and in MEM with transferrin. After in vitro culture embryos were processed by routine histological methods and histologically evaluated. Embryos cultivated in medium supplemented with serum were used as a control. After 14 days in vitro rat embryos give arise to various differentiated tissues: epithelia, neuroblasts, myotubes and cartilage, depending on the added substances. Addition of FGF stimulates growth of whole explants and survival of neural tissue. In medium with EGF epidermis differentiates better than in control medium. Although transferin couldn't stimulate growth of whole embryos, it stimulates differentiation of neuroblasts (Fig. 1), columnar epithelium and cartilage.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA