Naslov
First Report on PVL-Positive Methicillin- Resistant Staphylococcus Aureus of Sccmec Type V, SPA Type T441 in Croatia

Autori
Budimir, Ana ; Tićac, Brigita ; Rukavina, Tomislav ; Farkaš, Maja ; Kalenić, Smilja

Izvornik
Collegium antropologicum (0350-6134) 40 (2016), 2; 133-137

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
MRSA ; Typing ; PVL ; community

Sažetak
In period from 2001 and 2007, all MRSA strains isolated from patients without risk factors for acquisition of HA-MRSA, or non-multiresistant profiles were collected and stored until further analysis. Strains originated mostly from non- infectious sites, majority ( N=20) was isolated from nasal swab, 15 from wound swabs, and two or one isolate per following sites: skin infection sample, furuncullus, conjunctiva, tongue. Wound swabs isolates are all collected later, 2004- 2007, while nasal swabs and other strains date from 2001-2004. All isolates were identified based on colony morphology, catalase, coagulase and DNA-se presence. Isolates were screened for MRSA with oxacillin, cefoxitin disk and latex agglutination test (BioMerieux). 46 MRSA strains were subjected to further analysis in Clinical Hospital Centre Zagreb, Department of Clinical and Molecular Microbiology and, to Mastricht for additionally tests. All isolates were mecA positive. SCCmec typing reveiled 26 MRSA strains with SCCmec type IV, three with SCCmec type V, and 13 had SCCmec I. SCCmec type II and III were not observed in this study. Four MRSA strains were non-typeable with applied SCCmec typing methods. PVL was detected in 4 strains, two SCCmec IV and two SCCmec V. PFGE analysis, using Tenover criteria, grouped MRSA strains into six similarity groups (A, B, C, D, E, F) containing 9, 9, 6, 2, 2 and 2 isolates, respectively, and 18 singletons. Dominating spa types in this collection of strains were t015, with 15 strains, followed by t041 (N=7), t051, (N=2 ), t2850 (N=2), t008 (N=2) and single isolates t441, t002, t448, t018, t019, t355, t390, t026, t449, t148. We also detected two new spa types, t3510 and t3509, respectively. Isolates were classified into 2 clusters: CC 1 with t008 t024 and t051, and other cluster, CC41 consisting of t002, t041 and t2027.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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