Naslov
Molecular detection of enteroviral RNA in cerebrospinal fluid

Autori
Planinić, Ana ; Grgić, Ivana ; Židovec-Lepej, Snježana ; Tešović, Goran ; Benić, Branka ; Miše, Branko ; Baće, Ana ; Markovinović, Leo ;

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
6th Croatian Congress on Infectious Diseases with International Participation ; 2009 Oct 24-2009 Oct 27 ; Šibenik. / - Samobor : HDI - Hrvatsko društvo za infektivne bolesti, 2009, 75-76

Skup
6th Croatian Congress on Infectious Diseases with International Participation

Mjesto i datum
Šibenik, Hrvatska, 24.10.2009. - 27.10.2009

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
enteroviral RNA ; cerebrospinal fluid ; aseptic meningitis ; real-time polymerase-chain reaction

Sažetak
Background: Detection of enteroviral RNA (EV RNA) in the cerebrospinal fluid (CSF) of patients with aseptic meningitis (AM) by real-time polymerase-chain reaction (PCR) has become a part of routine diagnostic work-up. Rapid detection of EV RNA enables physicians to diagnose patients with EV AM within a clinically relevant time-frame (usually 24 h). Objective: The aim of this study was to describe the monthly distribution of EV RNA positive real-time PCR results obtained from CSF samples taken at the University Hospital for Infectious Diseases in Zagreb (UHID) during 2008 season. Materials and methods: Detection of EV RNA in the CSF was performed by using a standardized pan-enteroviral real-time PCR Cepheid ASR Enterovirus assay (Cepheid, USA). CSF samples were tested for the presence of EV RNA as a part of routine diagnostics in pediatric patients with presumed diagnosis of AM. Results: Children aged 7 days to 13 years (median 7.2 years) were included in the study. Enteroviral RNA was detected in 38 out of 95 CSF samples (40 %) tested. The first patients with PCR-positive results were detected in April (n=2) and May (n=2). Between June and September, a total of 15 PCR-positive results were obtained. The highest number of PCR-positive results within one month were obtained during October (n=11) which can be attributed to a localized outbreak. Conclusion: Pan-enteroviral PCR-positive results can be found throughout the majority of the year (between April and December). PCR Cepheid ASR Enterovirus assay is suitable for rapid detection of EV RNA in the CSF of patients with clinical symptoms and laboratory parameters suggestive of AM.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti

POVEZANOST RADA