Naslov
Distribution of HLA microsatellite alleles in patients with testicular carcinoma

Autori
Štingl, Katarina ; Gotovac, Kristina ; Kaštelan, Željko ; Žunec, Renata ; Grubić, Zorana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Tissue Antigens / Mytilineos, Joannis ; Muller, Carlheinz ; Schrezenmeier, Hubert ; Blasczyk, Rainer - Ulm : Wiley-Blackwell, 2009, 505-505

Skup
23rd Immunogenetics and Histocompatibility Conference

Mjesto i datum
Ulm, Njemačka, 09.05.2009. - 12.05.2009

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
HLA microsatellite alleles; testicular carcinoma

Sažetak
Associations between particular HLA microsatellite alleles and carcinomas have reported by numerous scientific groups. In the present study, the distribution of HLA microsatellite alleles (D6S291, D6S1014, D6S273, TNFd, TNFa, TNFb, D6S2793, STR_MICA, D6S2927) was analysed in the group of 27 patients with testicular carcinoma and in two groups of healthy controls: K1-male and female (N=170) and K2-only male (N=45). Analysis was performed using PCR amplification with specific, fluorescently labelled primers and electrophoresis on a 6% polyacrylamide gel in an automated sequencer (ALFexpress, Amersham Pharmacia, Uppsala, Sweden). The comparison of the tested groups revealed that there is no significant difference in frequency distributions of alleles at TNFd and D6S2793 loci. Conversely, a statistically significant increase in the frequency among patients in comparison to controls was observed for the following alleles: D6S291-3 (K1-Pcorr=0.00007 ; K2-Pcorr=0.00035), TNFa4 (K1-Pcorr=0.00015 ; K2-Pcorr=0.00060) and TNFa5 (K1-Pcorr=0.00450 ; K2-Pcorr=0.00945. Taking the relative risk values of these alleles (D6S291-3: RR=4.20 and RR=6.89 ; TNFa4: RR=6.40 and RR=9.05 ; and TNFa5: RR=4.00 and R=6.82) suggest that they could be considered susceptibility alleles for developing testicular carcinoma. A statistically significant decrease in frequency of TNFa10, D6S291-1 and TNFb4 alleles was observed in patient group in comparison to controls. This may imply a protective role of the alleles in question in aetiology of testicular carcinoma. These results provide basis for all further studies about correlation between HLA region and testicular carcinoma, which should be performed on a larger patient group and include additional genetic markers in this region.Associations between particular HLA microsatellite alleles and carcinomas have reported by numerous scientific groups. In the present study, the distribution of HLA microsatellite alleles (D6S291, D6S1014, D6S273, TNFd, TNFa, TNFb, D6S2793, STR_MICA, D6S2927) was analysed in the group of 27 patients with testicular carcinoma and in two groups of healthy controls: K1-male and female (N=170) and K2-only male (N=45). Analysis was performed using PCR amplification with specific, fluorescently labelled primers and electrophoresis on a 6% polyacrylamide gel in an automated sequencer (ALFexpress, Amersham Pharmacia, Uppsala, Sweden). The comparison of the tested groups revealed that there is no significant difference in frequency distributions of alleles at TNFd and D6S2793 loci. Conversely, a statistically significant increase in the frequency among patients in comparison to controls was observed for the following alleles: D6S291-3 (K1-Pcorr=0.00007 ; K2-Pcorr=0.00035), TNFa4 (K1-Pcorr=0.00015 ; K2-Pcorr=0.00060) and TNFa5 (K1-Pcorr=0.00450 ; K2-Pcorr=0.00945. Taking the relative risk values of these alleles (D6S291-3: RR=4.20 and RR=6.89 ; TNFa4: RR=6.40 and RR=9.05 ; and TNFa5: RR=4.00 and R=6.82) suggest that they could be considered susceptibility alleles for developing testicular carcinoma. A statistically significant decrease in frequency of TNFa10, D6S291-1 and TNFb4 alleles was observed in patient group in comparison to controls. This may imply a protective role of the alleles in question in aetiology of testicular carcinoma. These results provide basis for all further studies about correlation between HLA region and testicular carcinoma, which should be performed on a larger patient group and include additional genetic markers in this region.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

POVEZANOST RADA