Naslov
Molekularna dijagnostika infekcije virusom hepatitisa C i hepatitisa B
(Molecular diagnosis of hepatitis C and hepatitis B infection)

Autori
Židovec-Lepej, Snježana ; Dušek, Davorka ; Budimir, Jelena ; Vince, Adriana

Izvornik
Acta medica Croatica (1330-0164) 63 (2009), 5; 361-369

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, stručni

Ključne riječi
*
(Genotype; Hepatitis B; Hepatitis C; Molecular methods; PCR assays)

Sažetak
Molecular methods are a well-established part of routine diagnostic work-up in patients infected with hepatitis B virus (HBV) and hepatitis C virus (HCV). Confirmation of active viral replication in infected patients is based on detection and/or quantification of viral genome in serum by molecular assays. Diagnostic algorithm for hepatitis C includes detection and/or quantification of HCV RNA in serum of infected patients and HCV genotyping. Diagnostic work-up in patients with hepatitis B includes quantification of HBV DNA in serum, HBV genotyping, and determination of resistance to nucleoside and nucleotide analogues. Real-time polymerase chain reaction (PCR) is the standard recommended molecular method for quantification of HCV RNA and HBV DNA in clinical samples. Due to superior sensitivity real-time PCR assays can provide both qualitative detection of viral genome and quantification. Molecular diagnosis of HCV and HBV infections in clinical laboratories should be limited to certified standardized assays.

Izvorni jezik
Hrvatski

Znanstvena područja
Kliničke medicinske znanosti

POVEZANOST RADA