Naslov
Analysis of metagenomes in extreme environments

Autori
Roller Milošević, Maša

Vrsta, podvrsta i kategorija rada
Ocjenski radovi, diplomski rad

Fakultet
Prirodoslovno-matematički fakultet

Mjesto
Zagreb

Datum
09.09

Godina
2009

Stranica
33

Mentor
Vlahoviček, Kristian

Ključne riječi
metagenomics; codon usage bias; extreme environments

Sažetak
Not all prokaryotes are amenable to cultivation in laboratory conditions, and increasing amounts of knowledge about microbial diversity is gained from direct sampling of DNA from environments. A rapidly developing field that studies whole microbial communities is metagenomics, the culture-independent genomic study of organisms extracted directly from an ecological niche. Prokaryotic genomes show strong codon usage bias (CUB). CUB is directly correlated with expression levels of genes because codons used by genes expressed at high levels are encoded for by the most abundant tRNAs. Therefore, the CUB of a gene can be linked to its expression level through translational optimization. We show that metagenomes, much like genomes, also show CUB and that this phenomenon can be used to predict the expression level of genes at the level of the entire microbial community. We analyzed adaptation of organisms in metagenomes to their extreme environments through this approach.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Računarstvo

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