Naslov
Urine Immunocytology as a Noninvasive Diagnostic Tool for Acute Kidney Rejection: A Single Center Experience

Autori
Mihovilović, Karlo ; Kardum-Skelin, Ika ; Jelić Puškarić, Biljana ; Ljubanović, Danica ; Bulimbašić, Stela ; Sabljar-Matovinović, Mirjana ; Kovačević-Vojtušek, Ivana ; Gracin, Sonja ; Kocman, Branislav ; Jadrijević, Stipe ; Vidas, Željko ; Guštin, Denis ; Knotek, Mladen

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Knjiga sažetaka, 4. Hrvatski kongres kliničke citologije, 1. Hrvatski simpozij analitičke citologije i 2. Hrvatski simpozij citotehnologije s međunarodnim sudjelovanjem / Kardum-Skelin, Ika ; Batinić, Drago ; Anić, Veronika - Zagreb : Hrvatsko društvo za kliničku citologiju ; Hrvatska udruga citotehnologa, 2009

Skup
4. Hrvatski kongres kliničke citologije, 1. Hrvatski simpozij analitičke citologije i 2. Hrvatski simpozij citotehnologije s međunarodnim sudjelovanjem

Mjesto i datum
Split, Hrvatska, 11.10.2009. - 14.10.2009

Vrsta sudjelovanja
Pozvano predavanje

Vrsta recenzije
Nije recenziran

Ključne riječi
renal transplantation; acute rejection; immunocytology; urinary sediment

Sažetak
Aim - Renal biopsy is gold standard for diagnosing acute renal allograft rejection. Acute rejection may be associated with lymphocyte shedding in the urine. The aim of the present study was to evaluate diagnostic performance of urine immunocytology for CD3-positive cells in diagnosing renal allograft rejection. Participants and methods - This was a prospective single centre study performed in Clinical Hospital “Merkur”, Croatia. 54 kidney and kidney-pancreas transplant patients with 70 kidney biopsies (for cause or by protocol) and simultaneous urine immunocytologies (immunostaining for CD3) were included. Results - There were 24 AR cases, while in 46 biopsies AR was absent. Urine sediment was positive for CD3+ lymphocytes in 5 cases of AR (21%) and in 6 cases without AR (13%). CD3 positivity had sensitivity of 21%, specificity of 87%, positive predictive value of 45% and negative predictive value of 68% for diagnosis of AR. Discussion - These results demonstrate insufficient both sensitivity and specificity of urine immunocytology for CD3 for establishing diagnosis of renal AR. With respect to sensitivity our results are at odd with some of the previously published studies. Reasons for that are unclear, but may reflect either poor intrinsic performance of urine immunocytology for detection of AR, or may involve several technical factors like small initial volume of urine for analysis. Conclusion - Kidney biopsy still remains gold standard for detection AR. Urine immunocytology may have potential to become one of the methods for detection AR in kidney transplant patients, if sensitivity and specificity could be improved.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

POVEZANOST RADA