Naslov
Influence of flavonoids on Lck and Fyn tyrosine kinases using ELISA method with comparison of different substrates: Poly Glu:Tyr (4:1) and M3-01 decapeptide

Autori
Čalić, Maja ; Jelić, Dubravko ; Antolović, Roberto ; Verbanac, Donatella

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Congress of the Croatian Society of Biochemistry and Molecular Biology with international participation (HDBMB 2004) - Book of Abstracts / Dumić, Jerka ; Gornik, Olga - : Farmaceutsko-biokemijski fakultet Sveučilišta u Zagrebu, 2004, 69-69

ISBN
953-6256-44-4

Skup
Congress of the Croatian Society of Biochemistry and Molecular Biology with international participation (HDBMB 2004)

Mjesto i datum
HOC Bjelolasica, Hrvatska, 30.09.2004. - 02.10.2004

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
tyrosine kinases; Lck; Fyn; flavonoids; ELISA

Sažetak
Tyrosine phosphorylation represents unique signalling process in the cell as an answer to different extracellular signals. The enzymes that carry out this modification are protein tyrosine kinases (PTKs) which catalyse transfer of γ-phosphates of ATP on phenole -OH group of tyrosine of protein substrates. The largest subfamily of nonreceptor PTKs is the Src-family. Human Fyn and Lck tyrosine kinases are two of the nonreceptor Src-family tyrosine kinases involved in T-cell signalling transport. Flavonoids are biologically active polyphenole compounds naturally occuring in many plants. This group of compounds has been recognized as inhibitor of Fyn and Lck protein kinases. In conducted experiments, myricetin showed the highest inhibitory effect, with ATP non-competitive mechanism of inhibition. In contrast, competition with ATP has been proved in the example of staurosporine. Inhibitory activity of flavonoids on Fyn and Lck kinases was measured by ELISA (Enzyme-Linked Immunosorbent Assay) method. Affinity of these enzymes on two different substrates, polypeptide polymer PolyGlu:Tyr (4:1) and decapeptide M3-01 was tested. Development of efficient protein kinase inhibitors is important for the treatment of diseases, but also as a tool to investigate the physiological roles of protein kinases.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Temeljne medicinske znanosti

POVEZANOST RADA