Naslov
SURVEY OF PHYTOPLASMA DIVERSITY IN HEAVILY GRAPEVINE YELLOWS AFFECTED AREAS OF CROATIA

Autori
Šeruga Musić, Martina ; Budinščak, Željko ; Križanac, Ivana ; Mikec, Ivan ; Škorić, Dijana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstract book of the scientific meeting of the COST Action FA0807 Integrated Management of Phytoplasma Epidemics in Different Crop Systems: "Current status and perspective of phytoplasma disease research and management" / Bertaccini, Assunta ; Lavina, Amparo ; Torres, Ester - , 2010, 35-35

Skup
Scientific meeting of the COST Action FA0807 Integrated Management of Phytoplasma Epidemics in Different Crop Systems: "Current status and perspective of phytoplasma disease research and management"

Mjesto i datum
Sitges, Španjolska, 01.02.2010. - 02.02.2010

Vrsta sudjelovanja
Pozvano predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Flavescence dorée; grapevine; PCR; real-time PCR; stolbur; weed

Sažetak
Surveys of grapevine yellows (GY) in Croatia are being conducted since 1997 with only Bois Noir (BN) and Aster Yellows (AY) phytoplasmas confirmed so far as the etiological agents of the disease. On the bases of visual surveys conducted from 2006 to 2009, Eastern Slavonia, Međimurje and Istria were assessed to be the most heavily affected by GY. Symptomatology, the geographical occurrence of the principal Flavescence dorée (FD) vector Scaphoideus titanus and distribution of FD in the neighboring countries lead us to believe that these areas are potentially under the most infective pressure from FD phytoplasmas. In the last 4 years, molecular analyses confirmed the presence of BN phytoplasmas in 47 out of 87 tested vines, with equal distribution of infected plants among the three regions. The identity of phytoplasmas was confirmed by sensitive triplex real-time PCR procedure simultanelusly detecting the presence of BN-P and FD-P map genes (Pelletier et al., Vitis, 48, 87-95. 2009), as well as the routine PCR-RFLP analyses. Only 1 out of 3 S. titanus from Istria in 2006 was found to carry phytoplasmas but of the AY type (16SrI-B). In the following years, 33 S. titanus samples from all regions tested negative for phytoplasma presence. Four out of ten Clematis vitalba samples were found positive for the presence of 16SrV group phytoplasma both in the PCR-RFLP and real-time PCR assays. Three of the positive C. vitalba plants were sampled in Međimurje and the other in Istria region. Since the high content of phenolic compounds in the plant tissues influence the detection sensitivity of PCR-based methods, we are currently testing the impact of the tissue type and the extraction method on the sensitivity of the real-time PCR phytoplasma detection. Aside from the classical CTAB-extraction method, various commercial kits for nucleic acid extraction are used. Preliminary results of the detection protocol comparisons will be presented as well as the new findings on GY phytoplasma diversity in grapevines, insects and weeds from 2009.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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