Naslov
Modulation of thymic and hepatic heat shock protein gp96 expression by disturbance of morhostasis

Autori
Jakovac, Hrvoje ; Mrakovčić-Šutić, Ines ; Radošević-Stašić, Biserka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Ljubljana 2010, Abstracts / Schoenfeld Yehuda - Ženeva : C/o Kenes International, 2010, Abstract 844-.

Skup
7th International Congress on Autoimmunity

Mjesto i datum
Ljubljana, Slovenija, 05.05.2010. - 09.05.2010

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
gp96; liver regeneration; thymus; liver; spleen.

Sažetak
Background: Heat shock protein gp96, has been reported to play important role in the endoplasmic reticulum stress response, acting as a molecular chaperone in the unfolded protein response. Additionally, owing to it’s ability to bind protein and nonprotein molecules with exposed hydrophobic residua, it’s function in antigen presentation and cross-presentation, activation of macrophages and lymphocytes, and activation and maturation of dendritic cells has been also proposed Material and methods: In this study we analyzed the expression of gp96 in the regenerating liver, thymus and spleen after 1/3 partial hepatectomy (pHx) in C57/Bl6 mice. The data, obtained by immunohistochemistry and quantitative real-time PCR, were correlated with phenotype and cytotoxicity of hepatic and splenic mononuclear lymphatic cells against NKT and NK-sensitive targets, as well as with the intensity of apoptosis in the liver and thymus. Results: High time-dependent changes were found in the expression of gp96 protein and mRNA in regenerating liver, thymus, and spleen. Simultaneously, in the liver and thymus augmented the proportion of autoreactive NKT and regulatory T cells, increased the cytotoxicity against the syngeneic thymocytes and augmented the apoptosis in the liver and thymus. Additionally, in thymus and spleen a marked upregulation of TLR2 was noticed. Conclusions: The data suggest that after tissue damages and disturbance of morphostasis gp96 may serve as a natural adjuvant for chaperoning antigenic self peptides and nonprotein molecules into the immune surveillance pathways, resulting in activation of autoreactive NKT and Treg cells that might participate in reestablishment of self tolerance.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA