Naslov
The positive influence of electrochemical cyclic potentiodynamic passivation (CPP) of a SS316LS surface on its response to fibronectin and pre-osteoblasts
(The positive Influence of Cyclic Potentiodynamic Polarization (CPP) Passivation of the SS316LS Surface on its Response to Fibronectin and Pre-osteoblasts)

Autori
Shahryari, Arash ; Azari, Fereshteh ; Vali, Hojatollah ; Omanovic, Sasha

Izvornik
Physical Chemistry Chemical Physics (1463-9076) 11 (2009), 29; 6218-6224

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
stainless steel SS316LS ; pre-osteoblasts ; fibronectin ; electrochemical passivation ; adsorption

Sažetak
The influence of an electrochemical surface passivation technique (cyclic potentiodynamic polarization, CPP) on the physico-chemical surface properties of SS316LS and its subsequent response to fibronectin (Fn) and pre-osteoblasts were investigated. Contact angle and zeta-potential measurements showed that the CPP-modified surface is more hydrophilic and more positively charged than the unmodified surface. Polarization modulation infrared reflection absorption spectroscopy (PM-IRRAS) was used to investigate the interaction of Fn with both surfaces. The saturated surface concentration of adsorbed Fn was higher on the CPP-modified surface. As well, significant changes were identified in the secondary structure of Fn adsorbed on both surfaces, in comparison to its native state. This data also indicated a higher degree of Fn unfolding on the CPP-modified surface. Cell studies indicated that the attachment, proliferation and morphology of pre-osteoblasts were significantly improved on the CPP-modified surface, which was attributed to the more open conformation of Fn on the CPP-modified surface. Thus, the CPP surface passivation method was demonstrated to yield a SS316LS surface of enhanced biocompatibility.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

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