Naslov
Transcription of pericentromeric heterochromatin in Palorus beetles - satellite DNAs as active regulatory elements

Autori
Pezer, Željka

Vrsta, podvrsta i kategorija rada
Ocjenski radovi, doktorska disertacija

Fakultet
Prirodoslovno-matematički

Mjesto
Zagreb

Datum
23.03

Godina
2010

Stranica
53

Mentor
Ugarković, Đurđica

Ključne riječi
centromere ; Coleoptera ; heterochromatin ; non-coding RNA ; satellite DNA ; transcription

Sažetak
PRAT and PSUB are major, highly abundant satellites in the heterochromatic regions of the beetles Palorus ratzeburgii and Palorus subdepressus, which are also found conserved in a low number of copies in the genomes of distant insect species. Extreme sequence conservation over a long evolutionary period indicates selective constraints and functional significance of these satellites. The comprehensive analysis of active transcription of PRAT and PSUB satellites is presented here, further supporting their functional importance. The results give strong indication that Palorus satellite DNAs are transcribed as autonomous transcription units from their own promoters that reside within satellite sequences. Long satellite DNA transcripts are proposed to play a structural role in the organization of pericentromeric heterochromatin.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA