Naslov
Cryopreserved human monocytes for the in vitro test of monocyte spreading inhibition

Autori
Martinić, Roko ; Svoboda-Beusan, Ivna ; Silobrčić, Vlatko

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
7. jugoslovanski simpozij imunologov : zbornik radova ; u: Perioducum biologorum 85 (S3) (3) / - Ljubljana : Slovensko imunološko društvo : Inštitut za mikrobiologijo Medicinske fakultete Univerze Edvarda Kardelja, 1983, 93-93

Skup
Jugoslovanski simpozij imunologov (7 ; 1982 ; Radenci)

Mjesto i datum
Radenci, Jugoslavija, 03.11.1982. - 06.11.1982

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
in vitro test of monocyte spreading inhibition ; cryopreserved human monocytes

Sažetak
We used human peripheral blood monocytes as indicator cells for the in vitro test of cellular immunity. We tried simple cryopreservation method without gradual cooling and storage at -198 ºC and placed the cells directly at -80 ºC. We first determined the functionally test of monocyte spreading. Thawed monocytes spread less well than fresh cells, but after 12 weeks the percentage of spread cells fell below 50%. In evaluation of immunosuppressive potential of the horse-anti.human lymphocyte globulin the monocyte sporeading indices were somewhar lower after preservation, most likely due the lower spreading ability of the thawed monocytes. The cryopreserved cells were not usable in the test of monocyte spreading inhibition after preservation for more than threee weeks. They were in clumpd and the number of cells in the suspension decreased, so that it made their use impractical.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti

POVEZANOST RADA