Naslov
In vitro propagation of the Bishop’s Hat, Epimedium alpinum L.

Autori
Mihaljević, Snježana

Vrsta, podvrsta i kategorija rada
Poglavlja u knjigama, znanstveni

Knjiga
Horticulture in 21st Century

Urednik/ci
Abigail N Sampson

Izdavač
Nova Science Publishers

Grad
Haupauge (NY)

Godina
2010

Raspon stranica
193-199

ISBN
978-1-61668-582-9

Ključne riječi
barrenwort, bishop’s hat, Berberidaceae, Epimedium alpinum, immature seed, organogenesis, regeneration, tissue culture

Sažetak
The genus Epimedium (Bishop’s Hat, Barrenwort) in the family Berberidaceae comprises about 50 species of herbaceous perennials. A number of species, hybrids and cultivars are grown as ornamentals in woodland gardens and naturalized areas. Certain species are also in use in traditional Asian medicine. The plant can be propagated by division of its long rhizomes or by seeds. The difficulties associated with gathering the seeds and with slow growth make bishop’s hat a suitable species for propagation by tissue culture. In vitro propagation of Epimedium alpinum L. was carried out using organogenic callus induced from immature seeds transiently exposed (48 hours) to 20 µM CPPU or 80 µM TDZ followed by culture on hormone-free woody-plant medium (WPM). To promote adventitious shoot induction, the effects of different concentrations of 6-benzyladenine (BA) in combination with 2, 4-dichlorophenoxyacetic acid (2, 4-D) and gibberellic acid (GA3) were investigated. WPM containing 2.2 µM BA and 1.1 µM 2, 4-D was optimal for callus proliferation while shoot induction and elongation was enhanced on media with 44 µM BA and 0.55 µM 2, 4-D. Histological examination of the cultures revealed that the regenerated plants were derived from de novo developed shoots. Shoots were successfully rooted on hormone-free WPM and well-developed plantlets were transferred to soil. Somatic chromosome number of the regenerated plants was found to be the same as the source plants.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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