Naslov
Validation of an enzyme-linked immunosorbent assay for qualitative screening of neomycin in muscle, liver, kidney, eggs and milk

Autori
Solomun, Božica ; Bilandžić, Nina ; Varenina, Ivana ; Scortichini, Giampiero

Izvornik
Food additives and contaminants part A. Chemistry analysis control exposure & risk assessment (1944-0049) 28 (2011), 1; 11-18

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
neomycin ; muscle ; kidney ; liver ; eggs ; milk ; enzyme-linked immunosorbent assay

Sažetak
A rapid and sensitive enzyme-linked immunosorbent assay (ELISA) was used for the qualitative screening analysis of neomycin in food of animal origin (muscle, liver, kidney, eggs and milk) at level corresponding to the European Union (EU) maximum residue limit (MRL) set for this substance. The method validation was performed according to the criteria of Commission Decision 2002/657/EC established for qualitative screening methods. In this regard, the following parameters were determined: detection capability (CCβ), specificity, detection limit (LOD), quantitation limit (LOQ), recovery, within-laboratory reproducibility, linearity and ruggedness. LOD values ranged from 5.7 μg kg−1 in kidney to 29.3 μg kg−1 in milk and LOQ values ranged from 11.4 μg kg−1 in kidney to 59.7 μg kg−1 in eggs. The recoveries from spiked samples at the MRL, half the MRL and double the MRL levels ranged from 59.2% to 122.8%, with a coefficient of variation (CV) between 4.6% and 28.6%. The CCβ value was less than the MRL for all examined matrices. Moderate variations of some critical factors in the sample pre-treatment for muscle, milk and eggs were deliberately introduced for ruggedness evaluation and had a slight effect on method performance. The proposed method is suitable for qualitative screening analysis of neomycin in the above-mentioned food in conformity with current EU performance requirements.

Izvorni jezik
Engleski

Znanstvena područja
Javno zdravstvo i zdravstvena zaštita

POVEZANOST RADA