Naslov
Kinetics of tissue iron in experimental autoimmune encephalomyelitis in rats

Autori
Tota, Marin ; Jakovac, Hrvoje ; Grebić, Damir ; Marinić, Jelena ; Broznić, Dalibor ; Čanadi-Jurešić, Gordana ; Milin, Čedomila ; Radošević-Stašić, Biserka

Izvornik
Biological trace element research (0163-4984) 143 (2011), 1; 332-343

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
monophasic experimental autoimmune encephalomyelitis (EAE); chronic relapsing EAE; AO rats; DA rats; inductively coupled plasma spectrometry; iron; brain; spinal cord; liver.

Sažetak
To elucidate the role of iron in the pathomechanisms of autoimmune CNS disorders we estimated the tissue concentrations of Fe2+ in the brain, spinal cord (SC) and liver in monophasic and chronic relapsing (CR) forms of experimental autoimmune encephalomyelitis (EAE). The diseases were induced in Albino Oxford (AO) and Dark Agouti (DA) strain of rats, by subcutaneous injection of myelin basic protein in complete Freund’s adjuvant (CFA) or by bovine brain homogenate in CFA, respectively. Control rats were treated with CFA or with saline solution. The data obtained by clinical assessment and by inductively coupled plasma spectrometry have shown that in both strains of rats the attacks of disease were followed by high accumulation of iron in the liver (on the 12th post-immunization day in AO rats and on the 12th and 22nd day in DA rats). Simultaneously, in AO rats decreased the concentration of Fe2+ in the brain (during the remission phase) and in the cervical spinal cord (during attack and remission). Similarly, in DA rats the lower iron content was found in cervical spinal cord during the second attack of disease. The data point to regulatory effects of iron and hepatic trace elements regulating mechanisms in the pathogenesis of EAE.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA