Naslov
MDA and 8-OHdG in urine of elderly persons from two regions in Croatia with different diet habits

Autori
Peraica, Maja ; Domijan, Ana-Marija ; Flajs, Dubravka ; Pavlović, Mladen

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Toxicology Letters, Vol.189, Supl. 1 - Abstracts of the 46th Congress of the European Societies of Toxicology / Toth, Heidi - Amsterdam : Elsevier, 2009, 119-119

Skup
46th European Congress of The European Societies of Toxicology

Mjesto i datum
Dresden, Njemačka, 13.09.2009. - 15.09.2009

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
elderly persons; malondialdehyde; 8-OHdG

Sažetak
Free radicals and other reactive species are continuously generated in human organism causing oxidative damage. One hypothesis of aging proposes that physiological changes of aging and higher rate of cancer are the consequence of the accumulation of random oxidative damage to DNA, lipids and proteins. It seems that the longevity and healthy aging in Mediterranean countries is the consequence of nutrition that contains natural antioxidants. The hypothesis of this study was that elderly residents on the Mediterranean part of Croatia have lower parameters of oxidative stress as compared to residents on the continental part. In this study urine and blood samples were collected from apparently healthy residents of the Mediterranean (Dalmatia region, N = 22) and continental part of Croatia (Virovitica and Zagreb regions, N = 15) aged more than 74 years. From this study all persons having some acute and chronic disease, and persons with abnormal laboratory blood and urine findings, were excluded. The concentrations of malondialdehyde (MDA) and 8-hydroxy-2′-deoxyguanosine (8-OHdG) were analyzed using HPLC with UV–vis and EC detectors, respectively. The concentration of MDA in urine of residents in continental part of Croatia (0.791 ± 0.106 mmol/L ; mean ± SEM) was significantly higher (P < 0.05) than in the residents of Mediterranean part (0.481 ± 0.075 mmol/L), while the concentration of 8-OHdG in urine was higher in continental part, but this difference was not significant (3.930 ± 0.644 nmol/L and 3.490 ± 0.593 nmol/L, respectively). These results indicate that Mediterranean type of nutrition may have some beneficial effects in ageing.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne tehničke znanosti, Temeljne medicinske znanosti, Kliničke medicinske znanosti

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