ࡱ> JLIq`bjbjqPqP .,::8888$\9n8888888$|:h<z888G!G!G! 8G!8G!G!!664x @ 8Z688096^=!(^=,66x^=i8$hJG!A<}h88=! 9  $  HYPERLINK "javascript:AL_get(this,%20'jour',%20'Diagn%20Microbiol%20Infect%20Dis.');" \o "Diagnostic microbiology and infectious disease." Diagn Microbiol Infect Dis. 2009 Dec;65(4):384-91. Epub 2009 Sep 24. A rapid, 2-well, multiplex real-time polymerase chain reaction assay for the detection of SCCmec types I to V in methicillin-resistant Staphylococcus aureus.  HYPERLINK "http://www.ncbi.nlm.nih.gov/pubmed?term=%22Valvatne%20H%22%5BAuthor%5D" Valvatne H,  HYPERLINK "http://www.ncbi.nlm.nih.gov/pubmed?term=%22Rijnders%20MI%22%5BAuthor%5D" Rijnders MI,  HYPERLINK "http://www.ncbi.nlm.nih.gov/pubmed?term=%22Budimir%20A%22%5BAuthor%5D" Budimir A,  HYPERLINK "http://www.ncbi.nlm.nih.gov/pubmed?term=%22Boumans%20ML%22%5BAuthor%5D" Boumans ML,  HYPERLINK "http://www.ncbi.nlm.nih.gov/pubmed?term=%22de%20Neeling%20AJ%22%5BAuthor%5D" de Neeling AJ,  HYPERLINK "http://www.ncbi.nlm.nih.gov/pubmed?term=%22Beisser%20PS%22%5BAuthor%5D" Beisser PS,  HYPERLINK "http://www.ncbi.nlm.nih.gov/pubmed?term=%22Stobberingh%20EE%22%5BAuthor%5D" Stobberingh EE,  HYPERLINK "http://www.ncbi.nlm.nih.gov/pubmed?term=%22Deurenberg%20RH%22%5BAuthor%5D" Deurenberg RH. Department of Medical Microbiology, Maastricht University Medical Center, Maastricht, The Netherlands. For us to assess the spread of methicillin-resistant Staphylococcus aureus (MRSA), typing of the staphylococcal cassette chromosome mec (SCCmec) is a valuable addition to existing typing methods, such as multilocus sequence typing (MLST). Traditional SCCmec typing assays, that is, that of Oliveira et al. and Ito et al., are polymerase chain reaction (PCR) based, requiring electrophoresis. We introduce a rapid, 2-well, multiplex real-time PCR assay that can be used directly on bacterial suspensions and is able to characterize SCCmec type I to V based on the detection of the ccr genes and the mec complex. The assay was evaluated on 212 clinical MRSA isolates from various countries, associated with MLST clonal complexes (CC) 1, 5, 8, 22, 30, and 45, as well as pig-associated CC398. When comparing the real-time PCR assay with traditional methods, the correct SCCmec element was identified in 209 (99%) of the 212 MRSA isolates. The new assay enables high-throughput analyses for SCCmec on large strain collections. PMID: 19781888 [PubMed - indexed for MEDLINE]  HYPERLINK "http://www.ncbi.nlm.nih.gov/pubmed/19781888" \l "#" \o "Supplemental information" Publication Types, MeSH Terms, Substances Publication Types:  HYPERLINK "javascript:AL_get(this,%20'ptyp',%20'Comparative%20Study');" Comparative Study  HYPERLINK "javascript:AL_get(this,%20'ptyp',%20'Evaluation%20Studies');" Evaluation Studies MeSH Terms:  HYPERLINK "javascript:AL_get(this,%20'mesh',%20'Animals');" Animals  HYPERLINK "javascript:AL_get(this,%20'mesh',%20'Bacterial%20Typing%20Techniques/methods*');" Bacterial Typing Techniques/methods*  HYPERLINK "javascript:AL_get(this,%20'mesh',%20'Bacteriological%20Techniques/methods*');" Bacteriological Techniques/methods*  HYPERLINK "javascript:AL_get(this,%20'mesh',%20'DNA,%20Bacterial/genetics*');" DNA, Bacterial/genetics*  HYPERLINK "javascript:AL_get(this,%20'mesh',%20'Genotype');" Genotype  HYPERLINK "javascript:AL_get(this,%20'mesh',%20'Humans');" Humans  HYPERLINK "javascript:AL_get(this,%20'mesh',%20'Methicillin-Resistant%20Staphylococcus%20aureus/classification');" Methicillin-Resistant Staphylococcus aureus/classification  HYPERLINK "javascript:AL_get(this,%20'mesh',%20'Methicillin-Resistant%20Staphylococcus%20aureus/genetics');" Methicillin-Resistant Staphylococcus aureus/genetics  HYPERLINK "javascript:AL_get(this,%20'mesh',%20'Methicillin-Resistant%20Staphylococcus%20aureus/isolation%20&%20purification*');" Methicillin-Resistant Staphylococcus aureus/isolation & purification*  HYPERLINK "javascript:AL_get(this,%20'mesh',%20'Polymerase%20Chain%20Reaction/methods*');" Polymerase Chain Reaction/methods*  HYPERLINK "javascript:AL_get(this,%20'mesh',%20'Sensitivity%20and%20Specificity');" Sensitivity and Specificity  HYPERLINK "javascript:AL_get(this,%20'mesh',%20'Staphylococcal%20Infections/microbiology*');" Staphylococcal Infections/microbiology*  HYPERLINK "javascript:AL_get(this,%20'mesh',%20'Staphylococcal%20Infections/veterinary*');" Staphylococcal Infections/veterinary*  HYPERLINK "javascript:AL_get(this,%20'mesh',%20'Swine%20Diseases/microbiology');" Swine Diseases/microbiology Substances:  HYPERLINK "javascript:AL_get(this,%20'subs',%20'DNA,%20Bacterial');" DNA, Bacterial  HYPERLINK "http://www.ncbi.nlm.nih.gov/pubmed/19781888" \l "#" \o "Links to resources such as full text articles and biological data" LinkOut - more resources r s + , 7 8 : ; X Y f g i j % & 4 5 7 8 45㺧hp^5CJ OJQJ\aJ %jhp^5CJ OJQJU\aJ hp^CJOJQJaJhp^5CJOJQJ\aJhp^0JCJOJQJaJhp^CJOJQJaJjhp^CJOJQJUaJ;r  41y__ & Fd-DM [$^gdp^``-D@&M gdp^dxx-D@&M gdp^d-DM gdp^d-DM gdp^d-DM gdp^d-DM gdp^ -DM gdp^d-DM gdp^ /012|}DEijkl>?WXYZɶ"hp^>*B*CJOJQJaJphhp^CJOJQJaJjhp^CJOJQJUaJ%hp^5B*CJOJQJ\aJph3fhp^5CJ OJQJ\aJ %jhp^5CJ OJQJU\aJ (hp^5>*B*CJOJQJ\aJph5kYA dxx-D@&M gdp^ & Fd-DM [$^gdp^ & Fd-DM [$^gdp^``-D@&M gdp^^_  ?@AB   jkcdghqr㫜hp^5CJ OJQJ\aJ %jhp^5CJ OJQJU\aJ %hp^5B*CJOJQJ\aJph3f"hp^>*B*CJOJQJaJphhp^CJOJQJaJjhp^CJOJQJUaJ:rh*hp^5CJ OJQJ\aJ %jhp^5CJ OJQJU\aJ (hp^5>*B*CJOJQJ\aJph,1h. 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