Naslov
Kinetic Spectrophotometric Determination of N-Acetyl-L-cysteine Based on a Coupled Redox-Complexation Reaction

Autori
Kukoč-Modun, Lea ; Radić, Njegomir

Izvornik
Analytical Sciences (0910-6340) 26 (2010), 4; 491-495

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
kinetic spectrophotometric determination; N-Acetyl-L-cysteine; pharmaceutical formulation

Sažetak
A novel simple kinetic spectrophotometric method for the determination of N-acetyl-L-cysteine (NAC) has been developed and validated. The proposed method is based on a coupled redox-complexation reaction, the first step of which is the reduction of Fe3+ by NAC ; the second one includes the complexation of Fe2+, resulting from the preceding redox reaction, with 2, 4, 6-trypyridyl-s-triazine (TPTZ). The stable Fe(TPTZ)2 2+ complex exhibits an absorption maximum at λ = 593 nm. The initial rate and fixed-time (at 5 min) methods were utilized for constructing calibration graphs. The graphs were linear in concentration ranges from 4.0 × 10–6 to 1.0 × 10–4 mol L–1 for the initial rate method and 1.0 × 10–6 to 1.0 × 10–4 mol L–1 for the fixed-time method, with detection limits of 1.0 × 10–6 mol L–1 and 1.7 × 10–7 mol L–1, respectively. The proposed methods were successfully applied for the determination of NAC in its commercial pharmaceutical formulations.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

POVEZANOST RADA