Naslov
The role of flanking sequences in antibody recognition of PTGTQ epitope of mucin-2

Autori
Windberg, Emöke ; Jelić, Dubravko ; Skribanek, Zsuzsi ; Uray, Katalyn ; Price, M.R. ; Sebestyén, Ferenc ; Hudecz, Ferenc

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
Journal of Peptide Science, Volume 6, Issue S1 (p S201-S210) / European Peptide Society and John Wiley & Sons, Ltd. - Pariz : Edition EDK, Paris/France, 2000, S210-S210

Skup
Peptides 2000 (26th European Peptide Symposium)

Mjesto i datum
Montpellier, Francuska, 10.09.2000. - 15.09.2000

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
antibody recognition; epitope; mucin-2

Sažetak
An epitope motif, TX1TX2T, of mucin-2 glycoprotein was identified by means of a mucin-2 specific monoclonal antibody, mAb 994 raised against a synthetic mucin-derived 15-mer peptide-conjugate. For determination of the epitope sequence recognized with highest affinity by mAb 994, a combinatorial approach was applied using the portioning-mixing technique excluding Cys. Antibody binding of libraries was most profound when Gln was at the X1 position.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Temeljne medicinske znanosti

POVEZANOST RADA