Naslov
Chemical differentiation of Berberis croatica and B. vulgaris using HPLC fingerprinting

Autori
Zovko Končić, Marijana ; Kremer, Dario ; Schühly, Wolfgang ; Brantner, Adelheid ; Karlović, Ksenija ; Kalođera, Zdenka

Izvornik
Croatica chemica acta (0011-1643) 83 (2010), 4; 451-456

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, prethodno priopćenje, znanstveni

Ključne riječi
Berberis vulgaris; Berberis croatica; HPLC fingerprinting

Sažetak
Roots, twigs, leaves and fruits of two Berberis species that are present in Croatia, common barberry (B. vulgaris L.) and Croatian barberry (B. croatica Horvat), were investigated. The quantity of berberine was determined by RP-HPLC-DAD. It was found that the quantity of berberine was different in twigs, but not in roots of the two species. In addition to that, a HPLC method combined with Unweighted Pair Group Method with Arithmetic mean (UPGMA) was developed for species differentiation and compared to some previously described procedures. The results indicate that common and Croatian barberry are closely related species, but also that they can be differentiated according to their HPLC fingerprints under appropriate chromatographic conditions and UPGMA variable selection.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Farmacija

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