Pregled bibliografske jedinice broj: 44910
The protective role of cyclic AMP in acute hepatotoxicity in mouse
The protective role of cyclic AMP in acute hepatotoxicity in mouse // Knjiga sažetaka / Rabatić, Sabina ; Lučin, Pero (ur.).
Zagreb: Hrvatsko imunološko društvo, 1999. str. P-23 (predavanje, domaća recenzija, sažetak, znanstveni)
CROSBI ID: 44910 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
The protective role of cyclic AMP in acute hepatotoxicity in mouse
Autori
Aleksić, Joško ; Čulo, Filip
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Knjiga sažetaka
/ Rabatić, Sabina ; Lučin, Pero - Zagreb : Hrvatsko imunološko društvo, 1999, P-23
Skup
1999 Annual Meeting of Croatian Immunological Society
Mjesto i datum
Zagreb, Hrvatska, 25.10.1999
Vrsta sudjelovanja
Predavanje
Vrsta recenzije
Domaća recenzija
Ključne riječi
cyclic AMP; acute hepatotoxicity
Sažetak
Interleukin 1-alpha have protective effect on acetaminophen (APAP)-induced hepatotoxicity in mouse if given 2 or more hours before APAP. It significantly reduced mortality of mice and decreased transaminase level. As it is known that IL 1-alpha stimulates cAMP synthesis, we investigated whether this protection by IL 1-alpha is mediated by cAMP. We checked this by giving cAMP analog (dibutyryl-cAMP - dbcAMP) in mice intoxicated with APAP or blocator of cAMP synthesis (2, 3-dideoxyadenosine � DDA), and by measuring cAMP synthesis in liver homogenates of mice protected with IL 1-alpha. Mice were given phenobarbitone-sodium in drinking water during 7 days (300 mg/kg) in order to induce hepatic drug-metabolizing enzymes. Thereafter, mice were fasted overnight and received IL 1-alpha (2 hours before APAP) or dibutyryl cAMP (15-30 min before APAP i.p. After that APAP (250 or 300 mg/kg) was given by gastric tube. Animals were allowed water 4 hours later. The mortality of mice was followed for 2 days and serum aminotransferase levels were determined 24 hours after AAP administrations. Levels of cAMP were determined from liver homogenates taken 6 hours after AAP administration by RIA procedure. Results showed that administration of IL 1-alpha or dbcAMP reduced mortality of mice and decreased serum aminotransferase levels. Essentially, the opposite results were obtained with DDA. The results of cAMP levels in liver homogenates and slices are presently being investigated and results will be reported on the meeting.
Izvorni jezik
Engleski
Znanstvena područja
Farmacija
