Naslov
Kratki introni ukazuju na kompaktan genom kod spužava
(Short introns indicate a compact genome in sponges)

Autori
Štifanić, Mauro ; Batel, Renato

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
9. Croatian Biological Congress Proceeding of abstracts / Besendorfer, Višnja ; Klobučar, Goran, I, V - Zagreb : Hrvatsko biološko društvo, 2006, 64-65

ISBN
953-6241-06-4

Skup
9. Croatian Biological Congress

Mjesto i datum
Rovinj, Hrvatska, 23.09.2006. - 29.09.2006

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Domaća recenzija

Ključne riječi
intron; gene; genome; porifera; sponges

Sažetak
Sponges are among the oldest multicellular animals and, as such, are very interesting from an evolutionary point of view. Current knowledge of genome size and organisation in sponges is contradictory. The aim of this study was a statistical exon/intron size analysis in sponges, in order to conclude about their genome organisation. The NCBI nucleotide sequence database was searched for Poriferan protein-coding genomic sequences longer than 1000 nucleotides, excluding mitochondrial genes and patents. The retreaved sequences were then used to search for corresponding cDNAs using the BLASTN algorithm. We finally obtained 18 cDNA sequences that matched to 15 genomic clones. Here we present a statistical exon/intron size analysis of all sponge genes with both the CDS and the genomic sequences deposited in the NCBI sequence database at the time of writing. The analysis was based on all complete exons contained within the CDSs and all introns between the start and stop codons. The average size of 78 exons, and 96 introns is 146 nt with a median of 119, and 259 nt with a median of 181 respectively. The regions between the start and stop codons of the analysed genes contain a total of 19143 coding and 24822 noncoding nucleotides making a very high "CDSs size" vs. "introns contained within" ratio of 0.77. Based on the positive correlation of intron size and genome size, we conclude that sponges have a compact genome.

Izvorni jezik
Engleski

Znanstvena područja
Geologija, Biologija

POVEZANOST RADA