Naslov
Seryl-tRNA synthetases from methanogenic archaea: suppression of bacterial amber mutation and heterologous toxicity

Autori
Lesjak, Sonja ; Boko, Draško ; Weygand-Đurašević, Ivana

Izvornik
Food technology and biotechnology (1330-9862) 48 (2010), 4; 512-518

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
seryl-tRNA synthetase; heterologous toxicity; amber suppression; methanogenic archaea

Sažetak
Methanogenic archaea possess unusual seryl-tRNA synthetases (SerRS), evolutionarily distinct from the SerRSs found in other archaea, eucaryotes and bacteria. Our recent X-ray structural analysis of Methanosarina barkeri SerRS revealed an idiosyncratic N-terminal domain and catalytic zinc-ion in the active site. To shed further light on substrate discrimination by methanogenic-type SerRS, we set up to explore in vivo the interaction of methanogenic-type SerRSs with their cognate tRNAs in Escherichia coli or Saccharomyces cerevisiae. The expression of various methanogenic-type SerRSs was toxic for E. coli, resulting in synthesis of erroneous proteins, as revealed by beta-galactosidase stability assay. Although SerRSs from methanogenic archaea recognize tRNAsSer from all three domains of life in vitro, the toxicity presumably precluded the complementation of endogenous SerRS function in both E. coli and S. cerevisiae. However, despite the observed toxicity, co-expression of methanogenic-type SerRS with its cognate tRNA suppressed bacterial amber mutation.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija

POVEZANOST RADA